Comparative Transcriptome Analysis of CMV or 2b-Deficient CMV-Infected dcl2dcl4 Reveals the Effects of Viral Infection on Symptom Induction in Arabidopsis thaliana
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Growth and Virus Infection
2.2. Transcriptome Sequencing
2.3. Mapping and Quantification Differentially Expressed Genes (DEGs)
2.4. Gene Ontology (GO) Enrichment Analysis and Venn Analysis
2.5. Gene Expression Analysis
3. Results
3.1. Sequencing and De Novo Assembly of Transcriptome
3.2. Comparison of the Differential Transcriptome between CMV-Infected and Mock Plants
3.3. Asymptomatic Infections of CMV-Δ2b or CMV-2aTΔ2b in Col-0 Are in Relation to Cell Division and Cellular Metabolism
3.4. Disease Symptom of Col-CMV, dcl2/4-CMV, dcl2/4-Δ2b, and dcl2/4-2aTΔ2b Could Be Connected to Resistance Genes and Photosynthesis
3.5. Distinct Pathways Affected in Col-0 Infected by Intact CMV
3.6. Pathways Specifically Modified in Antiviral RNAi-Defecient dcl2/4 Mutant
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Xu, Q.; Shen, L.; Jin, L.; Wang, M.; Chang, F.; Guo, Z. Comparative Transcriptome Analysis of CMV or 2b-Deficient CMV-Infected dcl2dcl4 Reveals the Effects of Viral Infection on Symptom Induction in Arabidopsis thaliana. Viruses 2022, 14, 1582. https://doi.org/10.3390/v14071582
Xu Q, Shen L, Jin L, Wang M, Chang F, Guo Z. Comparative Transcriptome Analysis of CMV or 2b-Deficient CMV-Infected dcl2dcl4 Reveals the Effects of Viral Infection on Symptom Induction in Arabidopsis thaliana. Viruses. 2022; 14(7):1582. https://doi.org/10.3390/v14071582
Chicago/Turabian StyleXu, Qian, Li Shen, Liying Jin, Meng Wang, Fenghan Chang, and Zhongxin Guo. 2022. "Comparative Transcriptome Analysis of CMV or 2b-Deficient CMV-Infected dcl2dcl4 Reveals the Effects of Viral Infection on Symptom Induction in Arabidopsis thaliana" Viruses 14, no. 7: 1582. https://doi.org/10.3390/v14071582
APA StyleXu, Q., Shen, L., Jin, L., Wang, M., Chang, F., & Guo, Z. (2022). Comparative Transcriptome Analysis of CMV or 2b-Deficient CMV-Infected dcl2dcl4 Reveals the Effects of Viral Infection on Symptom Induction in Arabidopsis thaliana. Viruses, 14(7), 1582. https://doi.org/10.3390/v14071582