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Article

Monocyte Gene and Molecular Expression Profiles Suggest Distinct Effector and Regulatory Functions in Beninese HIV Highly Exposed Seronegative Female Commercial Sex Workers

1
Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), Montreal, QC H2X 0A9, Canada
2
Département de Microbiologie, Infectiologie et Immunologie de l‘Université de Montréal, Montreal, QC H3T 1J4, Canada
3
Département de Sciences de l’informatique, Université du Québec à Montréal, Montreal, QC H2X 3Y7, Canada
4
Service de Maladies Infectieuses, Hôpital Maisonneuve-Rosemont, Montreal, QC H1T 2M4, Canada
5
Centre de Recherche du CHU de Québec-Université Laval, Quebec, QC G1E 6W2, Canada
6
Département de Médecine Sociale et Préventive, Université Laval, Quebec, QC G1V 0A6, Canada
7
Institut National de Santé Publique du Québec, Quebec, QC H2P 1E2, Canada
8
Dispensaire IST, Cotonou, Benin
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Nicole F. Bernard, Keith Fowke and Julie Lajoie
Viruses 2022, 14(2), 361; https://doi.org/10.3390/v14020361
Received: 16 December 2021 / Revised: 4 February 2022 / Accepted: 6 February 2022 / Published: 10 February 2022
(This article belongs to the Special Issue Correlates of Immune Protection against HIV Infection)
We have previously reported that the female genital tract (FGT) of Beninese HIV highly-exposed seronegative (HESN) commercial sex workers (CSWs), presented elevated frequencies of a myeloid HLA-DR+CD14+CD11c+ population presenting “tolerogenic” monocyte derived dendritic cells (MoDC) features. In order to assess whether a differential profile of monocytes may be involved in the generation of these genital MoDCs, we have herein characterized the blood monocyte compartment of Beninese HESNs (HIV-uninfected ≥ 10 years CSWs) and relevant controls (HIV-uninfected 2.5–5 years CSWs herein termed “early HESNs”), HIV-infected CSWs, and low-risk HIV-uninfected women from the general population. Transcriptomic analyses by RNA-Seq of total sorted blood monocytes demonstrate that in comparison to the control groups, HESNs present increased expression levels of FCGR2C, FCAR, ITGAX, ITGAM, CR2, CD68, and CD163 genes, associated with effector functions. Moreover, we found increased expression levels of genes associated with protection/control against SHIV/HIV such as CCL3, CCL4, CCL5, BHLHE40, and TNFSF13, as well as with immune regulation such as IL-10, Ahr, CD83, and the orphan nuclear receptor (NR)4A1, NR4A2, and NR4A3. Through multicolor flow cytometry analyses, we noticed that the frequencies of intermediate and non-classical monocyte populations tended to be elevated in the blood of HESNs, and exhibited increased expression levels of effector CD16, CD11c, CD11b, as well as regulatory HLA-G, IL-10, and IFN-α markers when compared to HIV-uninfected women and/or HIV-infected CSWs. This profile is compatible with that previously reported in the FGT of HESNs, and likely confers an enormous advantage in their resistance to HIV infection. View Full-Text
Keywords: HIV; resistance; highly exposed seronegative (HESN); commercial sex workers; monocytes; effector functions; regulatory functions HIV; resistance; highly exposed seronegative (HESN); commercial sex workers; monocytes; effector functions; regulatory functions
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MDPI and ACS Style

Blondin-Ladrie, L.; Fourcade, L.; Modica, A.; Aranguren, M.; de Montigny, N.; Labbé, A.-C.; Alary, M.; Guédou, F.; Poudrier, J.; Roger, M. Monocyte Gene and Molecular Expression Profiles Suggest Distinct Effector and Regulatory Functions in Beninese HIV Highly Exposed Seronegative Female Commercial Sex Workers. Viruses 2022, 14, 361. https://doi.org/10.3390/v14020361

AMA Style

Blondin-Ladrie L, Fourcade L, Modica A, Aranguren M, de Montigny N, Labbé A-C, Alary M, Guédou F, Poudrier J, Roger M. Monocyte Gene and Molecular Expression Profiles Suggest Distinct Effector and Regulatory Functions in Beninese HIV Highly Exposed Seronegative Female Commercial Sex Workers. Viruses. 2022; 14(2):361. https://doi.org/10.3390/v14020361

Chicago/Turabian Style

Blondin-Ladrie, Laurence, Lyvia Fourcade, Alessandro Modica, Matheus Aranguren, Nicolas de Montigny, Annie-Claude Labbé, Michel Alary, Fernand Guédou, Johanne Poudrier, and Michel Roger. 2022. "Monocyte Gene and Molecular Expression Profiles Suggest Distinct Effector and Regulatory Functions in Beninese HIV Highly Exposed Seronegative Female Commercial Sex Workers" Viruses 14, no. 2: 361. https://doi.org/10.3390/v14020361

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