Next Article in Journal
Special Issue “Structural Variations and Molecular Genetics of Hepatitis Virus and Related Viruses”
Previous Article in Journal
Clinical Course of Infection and Cross-Species Detection of Equine Parvovirus-Hepatitis
Previous Article in Special Issue
Inhibition of Orbivirus Replication by Fluvastatin and Identification of the Key Elements of the Mevalonate Pathway Involved
 
 
Article

Serological Cross-Reactions between Expressed VP2 Proteins from Different Bluetongue Virus Serotypes

1
School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, UK
2
The Pirbright Institute, Surrey, Woking GU24 ONF, UK
3
UMR VIROLOGIE 1161, INRAE, Ecole Nationale Vétérinaire d’Alfort, ANSES, Université Paris-Est, F-94700 Maisons-Alfort, France
4
John Innes Centre, Department of Biochemistry and Metabolism, Norwich Research Park, Norwich NR4 7UH, UK
5
Pathology and Infectious Diseases, School of Veterinary Medicine, University of Surrey, Guildford GU2 7XH, UK
6
Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar 125004, India
*
Author to whom correspondence should be addressed.
Academic Editors: Noemí Sevilla, Verónica Martín and José M. Rojas
Viruses 2021, 13(8), 1455; https://doi.org/10.3390/v13081455
Received: 5 June 2021 / Revised: 14 July 2021 / Accepted: 19 July 2021 / Published: 26 July 2021
(This article belongs to the Special Issue Bluetongue Virus: Pathogenesis and Vaccines)
Bluetongue (BT) is a severe and economically important disease of ruminants that is widely distributed around the world, caused by the bluetongue virus (BTV). More than 28 different BTV serotypes have been identified in serum neutralisation tests (SNT), which, along with geographic variants (topotypes) within each serotype, reflect differences in BTV outer-capsid protein VP2. VP2 is the primary target for neutralising antibodies, although the basis for cross-reactions and serological variations between and within BTV serotypes is poorly understood. Recombinant BTV VP2 proteins (rVP2) were expressed in Nicotiana benthamiana, based on sequence data for isolates of thirteen BTV serotypes (primarily from Europe), including three ‘novel’ serotypes (BTV-25, -26 and -27) and alternative topotypes of four serotypes. Cross-reactions within and between these viruses were explored using rabbit anti-rVP2 sera and post BTV-infection sheep reference-antisera, in I-ELISA (with rVP2 target antigens) and SNT (with reference strains of BTV-1 to -24, -26 and -27). Strong reactions were generally detected with homologous rVP2 proteins or virus strains/serotypes. The sheep antisera were largely serotype-specific in SNT, but more cross-reactive by ELISA. Rabbit antisera were more cross-reactive in SNT, and showed widespread, high titre cross-reactions against homologous and heterologous rVP2 proteins in ELISA. Results were analysed and visualised by antigenic cartography, showing closer relationships in some, but not all cases, between VP2 topotypes within the same serotype, and between serotypes belonging to the same ‘VP2 nucleotype’. View Full-Text
Keywords: Bluetongue virus; BTV; orbivirus; orbivirus serotypes; cross-serotype antibodies; VP2; plant expressed proteins; antigenic cartography Bluetongue virus; BTV; orbivirus; orbivirus serotypes; cross-serotype antibodies; VP2; plant expressed proteins; antigenic cartography
Show Figures

Figure 1

MDPI and ACS Style

Fay, P.C.; Mohd Jaafar, F.; Batten, C.; Attoui, H.; Saunders, K.; Lomonossoff, G.P.; Reid, E.; Horton, D.; Maan, S.; Haig, D.; Daly, J.M.; Mertens, P.P.C. Serological Cross-Reactions between Expressed VP2 Proteins from Different Bluetongue Virus Serotypes. Viruses 2021, 13, 1455. https://doi.org/10.3390/v13081455

AMA Style

Fay PC, Mohd Jaafar F, Batten C, Attoui H, Saunders K, Lomonossoff GP, Reid E, Horton D, Maan S, Haig D, Daly JM, Mertens PPC. Serological Cross-Reactions between Expressed VP2 Proteins from Different Bluetongue Virus Serotypes. Viruses. 2021; 13(8):1455. https://doi.org/10.3390/v13081455

Chicago/Turabian Style

Fay, Petra C., Fauziah Mohd Jaafar, Carrie Batten, Houssam Attoui, Keith Saunders, George P. Lomonossoff, Elizabeth Reid, Daniel Horton, Sushila Maan, David Haig, Janet M. Daly, and Peter P. C. Mertens. 2021. "Serological Cross-Reactions between Expressed VP2 Proteins from Different Bluetongue Virus Serotypes" Viruses 13, no. 8: 1455. https://doi.org/10.3390/v13081455

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop