Next Article in Journal
Adeno-Associated Virus (AAV) Gene Delivery: Dissecting Molecular Interactions upon Cell Entry
Next Article in Special Issue
SARS-CoV-2 N Protein Targets TRIM25-Mediated RIG-I Activation to Suppress Innate Immunity
Previous Article in Journal
Binding of Nanoparticles Harboring Recombinant Large Surface Protein of Hepatitis B Virus to Scavenger Receptor Class B Type 1
Previous Article in Special Issue
Rationale and Criteria for a COVID-19 Model Framework
 
 
Article

Resveratrol and Pterostilbene Inhibit SARS-CoV-2 Replication in Air–Liquid Interface Cultured Human Primary Bronchial Epithelial Cells

1
Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands
2
Department of Biomedical Sciences of Cells & Systems, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands
3
Department of Pathology and Medical Biology, GRIAC Research Institute, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands
4
Department of Pediatrics, Beatrix Children’s Hospital, GRIAC Research Institute, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands
5
Department of Pulmonary Diseases, GRIAC Research Institute, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands
6
Department of Critical Care, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Concetta Castilletti, Luisa Barzon and Francesca Colavita
Viruses 2021, 13(7), 1335; https://doi.org/10.3390/v13071335
Received: 1 June 2021 / Revised: 7 July 2021 / Accepted: 8 July 2021 / Published: 10 July 2021
(This article belongs to the Special Issue SARS-CoV-2 Host Cell Interactions)
The current COVID-19 pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has an enormous impact on human health and economy. In search for therapeutic options, researchers have proposed resveratrol, a food supplement with known antiviral, anti-inflammatory, and antioxidant properties as an advantageous antiviral therapy for SARS-CoV-2 infection. Here, we provide evidence that both resveratrol and its metabolically more stable structural analog, pterostilbene, exhibit potent antiviral properties against SARS-CoV-2 in vitro. First, we show that resveratrol and pterostilbene antiviral activity in African green monkey kidney cells. Both compounds actively inhibit virus replication within infected cells as reduced virus progeny production was observed when the compound was added at post-inoculation conditions. Without replenishment of the compound, antiviral activity was observed up to roughly five rounds of replication, demonstrating the long-lasting effect of these compounds. Second, as the upper respiratory tract represents the initial site of SARS-CoV-2 replication, we also assessed antiviral activity in air–liquid interface (ALI) cultured human primary bronchial epithelial cells, isolated from healthy volunteers. Resveratrol and pterostilbene showed a strong antiviral effect in these cells up to 48 h post-infection. Collectively, our data indicate that resveratrol and pterostilbene are promising antiviral compounds to inhibit SARS-CoV-2 infection. Because these results represent laboratory findings in cells, we advocate evaluation of these compounds in clinical trials before statements are made whether these drugs are advantageous for COVID-19 treatment. View Full-Text
Keywords: resveratrol; pterostilbene; SARS-CoV-2; antiviral; human primary bronchial epithelial cells resveratrol; pterostilbene; SARS-CoV-2; antiviral; human primary bronchial epithelial cells
Show Figures

Figure 1

MDPI and ACS Style

ter Ellen, B.M.; Dinesh Kumar, N.; Bouma, E.M.; Troost, B.; van de Pol, D.P.I.; van der Ende-Metselaar, H.H.; Apperloo, L.; van Gosliga, D.; van den Berge, M.; Nawijn, M.C.; van der Voort, P.H.J.; Moser, J.; Rodenhuis-Zybert, I.A.; Smit, J.M. Resveratrol and Pterostilbene Inhibit SARS-CoV-2 Replication in Air–Liquid Interface Cultured Human Primary Bronchial Epithelial Cells. Viruses 2021, 13, 1335. https://doi.org/10.3390/v13071335

AMA Style

ter Ellen BM, Dinesh Kumar N, Bouma EM, Troost B, van de Pol DPI, van der Ende-Metselaar HH, Apperloo L, van Gosliga D, van den Berge M, Nawijn MC, van der Voort PHJ, Moser J, Rodenhuis-Zybert IA, Smit JM. Resveratrol and Pterostilbene Inhibit SARS-CoV-2 Replication in Air–Liquid Interface Cultured Human Primary Bronchial Epithelial Cells. Viruses. 2021; 13(7):1335. https://doi.org/10.3390/v13071335

Chicago/Turabian Style

ter Ellen, Bram M., Nilima Dinesh Kumar, Ellen M. Bouma, Berit Troost, Denise P.I. van de Pol, Heidi H. van der Ende-Metselaar, Leonie Apperloo, Djoke van Gosliga, Maarten van den Berge, Martijn C. Nawijn, Peter H.J. van der Voort, Jill Moser, Izabela A. Rodenhuis-Zybert, and Jolanda M. Smit. 2021. "Resveratrol and Pterostilbene Inhibit SARS-CoV-2 Replication in Air–Liquid Interface Cultured Human Primary Bronchial Epithelial Cells" Viruses 13, no. 7: 1335. https://doi.org/10.3390/v13071335

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop