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Article

Fast Detection of SARS-CoV-2 RNA Directly from Respiratory Samples Using a Loop-Mediated Isothermal Amplification (LAMP) Test

1
Institute for Virology, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany
2
Labor Dr. Krause und Kollegen MVZ GmbH, 24106 Kiel, Germany
3
Institute of Virology, Medical Faculty, University Hospital Bonn, 53127 Bonn, Germany
4
Institute for Infection Medicine, Kiel University and University Medical Center Schleswig-Holstein, 24105 Kiel, Germany
*
Author to whom correspondence should be addressed.
These senior authors contributed equally to this study.
Academic Editors: Bertram Flehmig and Karin Klingel
Viruses 2021, 13(5), 801; https://doi.org/10.3390/v13050801
Received: 6 April 2021 / Revised: 23 April 2021 / Accepted: 26 April 2021 / Published: 29 April 2021
(This article belongs to the Special Issue Viral Markers and the Diagnosis of COVID-19)
The availability of simple SARS-CoV-2 detection methods is crucial to contain the COVID-19 pandemic. This study examined whether a commercial LAMP assay can reliably detect SARS-CoV-2 genomes directly in respiratory samples without having to extract nucleic acids (NA) beforehand. Nasopharyngeal swabs (NPS, n = 220) were tested by real-time reverse transcription (RT)-PCR and with the LAMP assay. For RT-PCR, NA were investigated. For LAMP, NA from 26 NPS in viral transport medium (VTM) were tested. The other 194 NPS were analyzed directly without prior NA extraction (140 samples in VTM; 54 dry swab samples stirred in phosphate buffered saline). Ten NPS were tested directly by LAMP using a sous-vide cooking unit. The isothermal assay demonstrated excellent specificity (100%) but moderate sensitivity (68.8%), with a positive predictive value of 1 and a negative predictive value of 0.65 for direct testing of NPS in VTM. The use of dry swabs, even without NA extraction, improved the analytical sensitivity; up to 6% of samples showed signs of inhibition. LAMP could be performed successfully with a sous-vide cooking unit. This technique is very fast, requires little laboratory resources, and can replace rapid antigen tests or verify reactive rapid tests on-site. View Full-Text
Keywords: SARS-CoV-2; COVID-19; RT-PCR; nucleic acids; direct testing; loop-mediated isothermal amplification; LAMP SARS-CoV-2; COVID-19; RT-PCR; nucleic acids; direct testing; loop-mediated isothermal amplification; LAMP
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MDPI and ACS Style

Anastasiou, O.E.; Holtkamp, C.; Schäfer, M.; Schön, F.; Eis-Hübinger, A.M.; Krumbholz, A. Fast Detection of SARS-CoV-2 RNA Directly from Respiratory Samples Using a Loop-Mediated Isothermal Amplification (LAMP) Test. Viruses 2021, 13, 801. https://doi.org/10.3390/v13050801

AMA Style

Anastasiou OE, Holtkamp C, Schäfer M, Schön F, Eis-Hübinger AM, Krumbholz A. Fast Detection of SARS-CoV-2 RNA Directly from Respiratory Samples Using a Loop-Mediated Isothermal Amplification (LAMP) Test. Viruses. 2021; 13(5):801. https://doi.org/10.3390/v13050801

Chicago/Turabian Style

Anastasiou, Olympia E., Caroline Holtkamp, Miriam Schäfer, Frieda Schön, Anna M. Eis-Hübinger, and Andi Krumbholz. 2021. "Fast Detection of SARS-CoV-2 RNA Directly from Respiratory Samples Using a Loop-Mediated Isothermal Amplification (LAMP) Test" Viruses 13, no. 5: 801. https://doi.org/10.3390/v13050801

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