Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters
Abstract
:1. Introduction
2. Material and Methods
2.1. Cloning, Generation and Purification of Adenoviral Vaccines
2.2. SARS-CoV-2 Virus and Cells
2.3. Animals and Vaccination
2.4. Infection Experiments
2.5. Virus Titrations, RNA Extractions and RT-qPCR
2.6. Serum Neutralization Tests (SNT)
2.7. T-Cell Restimulation Assays and Flow Cytometry
2.8. Histopathology
2.9. Statistics
3. Results
3.1. Course of Body Weight and Temperature after SARS-CoV-2 Exposure
3.2. Infectious SARS-CoV-2 Viruses and RNA Copies Isolated from Lung and Throat Swabs
3.3. Histopathology of Lung Tissue after Infection with SARS-CoV-2
3.4. Inflammation Score of Lung Histopathology
3.5. Course of Serum Neutralizing Antibody Titers after SARS CoV-2 Challenge
3.7. Comparison of T-Cell Reactivity in Mice after Vaccination with Ad-S, Ad-N and Ad-RBD
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Vaccine Gene | day 2 | day 5 | day 7 |
---|---|---|---|
N | 0 / 0 / 8 | 8 / 8 / 16 | 256 / 512 / 512 |
RBD | 16 / 32 / 32 | 256 / 512 / 512 | 512 / 512 / 512 |
S | 256 / 512 / 512 | 512 / 512 / 512 | 512 / 512 / 512 |
RBD-IRES-M | 256 / 256 / 512 | 64 / 512 / 512 | 512 / 512 / 512 |
mock-lacZ | 0 / 0 / 4 | 64 / 64 / 256 | 512 / 512 / 512 |
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Trimpert, J.; Herwig, S.; Stein, J.; Vladimirova, D.; Adler, J.M.; Abdelgawad, A.; Firsching, T.C.; Thoma, T.; Sehouli, J.; Osterrieder, K.; et al. Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters. Viruses 2021, 13, 2290. https://doi.org/10.3390/v13112290
Trimpert J, Herwig S, Stein J, Vladimirova D, Adler JM, Abdelgawad A, Firsching TC, Thoma T, Sehouli J, Osterrieder K, et al. Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters. Viruses. 2021; 13(11):2290. https://doi.org/10.3390/v13112290
Chicago/Turabian StyleTrimpert, Jakob, Susanne Herwig, Julia Stein, Daria Vladimirova, Julia M. Adler, Azza Abdelgawad, Theresa C. Firsching, Tizia Thoma, Jalid Sehouli, Klaus Osterrieder, and et al. 2021. "Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters" Viruses 13, no. 11: 2290. https://doi.org/10.3390/v13112290
APA StyleTrimpert, J., Herwig, S., Stein, J., Vladimirova, D., Adler, J. M., Abdelgawad, A., Firsching, T. C., Thoma, T., Sehouli, J., Osterrieder, K., Gruber, A. D., Sawitzki, B., Sander, L. E., & Cichon, G. (2021). Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters. Viruses, 13(11), 2290. https://doi.org/10.3390/v13112290