Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
AFM | acute flaccid myelitis |
ASU | Arizona State University |
COVID-19 | Coronavirus Disease 2019 |
EGT | enterovirus genotyping tool |
EV | enteroviruses |
EVA71 | enterovirus A71 |
HFMD | hand, foot, and mouth disease |
HHO | Human Health Observatory |
HTS | high-throughput sequencing |
ML | maximum-likelihood |
MSA | multiple sequence alignment |
ORF | open reading frame |
PEG | polyethylene glycol |
ppORF | polyprotein open reading frame |
RT-PCR | reverse transcription polymerase chain reaction |
SARS-CoV-2 | severe acute respiratory syndrome coronavirus 2 |
uORF | upstream open reading frame |
USA | United States of America |
WBE | wastewater-based epidemiology |
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Faleye, T.O.C.; Driver, E.; Bowes, D.; Adhikari, S.; Adams, D.; Varsani, A.; Halden, R.U.; Scotch, M. Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona. Viruses 2021, 13, 74. https://doi.org/10.3390/v13010074
Faleye TOC, Driver E, Bowes D, Adhikari S, Adams D, Varsani A, Halden RU, Scotch M. Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona. Viruses. 2021; 13(1):74. https://doi.org/10.3390/v13010074
Chicago/Turabian StyleFaleye, Temitope O. C., Erin Driver, Devin Bowes, Sangeet Adhikari, Deborah Adams, Arvind Varsani, Rolf U. Halden, and Matthew Scotch. 2021. "Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona" Viruses 13, no. 1: 74. https://doi.org/10.3390/v13010074