Next Article in Journal
Wide Diversity of Coronaviruses in Frugivorous and Insectivorous Bat Species: A Pilot Study in Guinea, West Africa
Previous Article in Journal
Chikungunya virus Detection in Aedes aegypti and Culex quinquefasciatus during an Outbreak in the Amazon Region
Open AccessArticle

Comprehensive Analyses of SARS-CoV-2 Transmission in a Public Health Virology Laboratory

1
Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, P.O. Box 5265601, Ramat-Gan, Israel
2
The Israel Center for Disease Control, Israel Ministry of Health, P.O. Box 5265601, Tel-Hashomer, Israel
3
Department of Epidemiology and Preventive Medicine, School of Public Health, Sackler Faculty of Medicine, Tel-Aviv University, P.O. Box 39040, Tel-Aviv, Israel
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2020, 12(8), 854; https://doi.org/10.3390/v12080854
Received: 9 July 2020 / Revised: 29 July 2020 / Accepted: 31 July 2020 / Published: 5 August 2020
(This article belongs to the Section Animal Viruses)
SARS-CoV-2 has become a major global concern as of December 2019, particularly affecting healthcare workers. As person-to-person transmission is airborne, crowded closed spaces have high potential for rapid virus spread, especially early in the pandemic when social distancing and mask wearing were not mandatory. This retrospective study thoroughly investigates a small-scale SARS-CoV-2 outbreak in Israel’s central virology laboratory (ICVL) in mid-March 2020, in which six staff members and two related family members were infected. Suspicions regarding infection by contaminated surfaces in ICVL facilities were nullified by SARS-CoV-2 negative real time polymerase chain reaction (PCR) of work surfaces swipe tests. Complete SARS-CoV-2 genomes were sequenced and mutation analyses showed inclusion of all samples to clades 20B and 20C, possessing the spike mutation D614G. Phylogenetic analysis clarified transmission events, confirming S1 as having infected at least three other staff members and refuting the association of a staff member’s infected spouse with the ICVL transmission cluster. Finally, serology tests exhibited IgG and IgA antibodies in all infected individuals and revealed the occurrence of asymptomatic infections in additional staff members. This study demonstrates the advantages of molecular epidemiology in elucidating transmission events and exemplifies the importance of good laboratory practice, distancing and mask wearing in preventing SARS-CoV-2 spread, specifically in healthcare facilities. View Full-Text
Keywords: 2019-nCoV; SARS-CoV-2; COVID-19; staff; infection; next generation sequencing (NGS) 2019-nCoV; SARS-CoV-2; COVID-19; staff; infection; next generation sequencing (NGS)
Show Figures

Figure 1

MDPI and ACS Style

Zuckerman, N.S.; Pando, R.; Bucris, E.; Drori, Y.; Lustig, Y.; Erster, O.; Mor, O.; Mendelson, E.; Mandelboim, M. Comprehensive Analyses of SARS-CoV-2 Transmission in a Public Health Virology Laboratory. Viruses 2020, 12, 854.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop