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Detection of Recombinant Rousettus Bat Coronavirus GCCDC1 in Lesser Dawn Bats (Eonycteris spelaea) in Singapore

Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814 USA
Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center–Frederick, Fort Detrick, MD 21702 USA
Leidos, Reston, VA 20190, USA
Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857, Singapore
Defense Threat Reduction Agency, Fort Belvoir, VA 22060 USA
Author to whom correspondence should be addressed.
Viruses 2020, 12(5), 539;
Received: 22 March 2020 / Revised: 10 May 2020 / Accepted: 12 May 2020 / Published: 14 May 2020
(This article belongs to the Special Issue Pathogenesis of Human and Animal Coronaviruses)
Rousettus bat coronavirus GCCDC1 (RoBat-CoV GCCDC1) is a cross-family recombinant coronavirus that has previously only been reported in wild-caught bats in Yúnnan, China. We report the persistence of a related strain in a captive colony of lesser dawn bats captured in Singapore. Genomic evidence of the virus was detected using targeted enrichment sequencing, and further investigated using deeper, unbiased high throughput sequencing. RoBat-CoV GCCDC1 Singapore shared 96.52% similarity with RoBat-CoV GCCDC1 356 (NC_030886) at the nucleotide level, and had a high prevalence in the captive bat colony. It was detected at five out of six sampling time points across the course of 18 months. A partial segment 1 from an ancestral Pteropine orthoreovirus, p10, makes up the recombinant portion of the virus, which shares high similarity with previously reported RoBat-CoV GCCDC1 strains that were detected in Yúnnan, China. RoBat-CoV GCCDC1 is an intriguing, cross-family recombinant virus, with a geographical range that expands farther than was previously known. The discovery of RoBat-CoV GCCDC1 in Singapore indicates that this recombinant coronavirus exists in a broad geographical range, and can persist in bat colonies long-term. View Full-Text
Keywords: lesser dawn bat; Rousettus bat coronavirus GCCDC1; recombinant; coronavirus lesser dawn bat; Rousettus bat coronavirus GCCDC1; recombinant; coronavirus
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Paskey, A.C.; Ng, J.H.J.; Rice, G.K.; Chia, W.N.; Philipson, C.W.; Foo, R.J.; Cer, R.Z.; Long, K.A.; Lueder, M.R.; Lim, X.F.; Frey, K.G.; Hamilton, T.; Anderson, D.E.; Laing, E.D.; Mendenhall, I.H.; Smith, G.J.; Wang, L.-F.; Bishop-Lilly, K.A. Detection of Recombinant Rousettus Bat Coronavirus GCCDC1 in Lesser Dawn Bats (Eonycteris spelaea) in Singapore. Viruses 2020, 12, 539.

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