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Open AccessArticle

Detection of Recombinant Rousettus Bat Coronavirus GCCDC1 in Lesser Dawn Bats (Eonycteris spelaea) in Singapore

1
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814 USA
2
Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center–Frederick, Fort Detrick, MD 21702 USA
3
Leidos, Reston, VA 20190, USA
4
Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857, Singapore
5
Defense Threat Reduction Agency, Fort Belvoir, VA 22060 USA
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(5), 539; https://doi.org/10.3390/v12050539
Received: 22 March 2020 / Revised: 10 May 2020 / Accepted: 12 May 2020 / Published: 14 May 2020
(This article belongs to the Special Issue Pathogenesis of Human and Animal Coronaviruses)
Rousettus bat coronavirus GCCDC1 (RoBat-CoV GCCDC1) is a cross-family recombinant coronavirus that has previously only been reported in wild-caught bats in Yúnnan, China. We report the persistence of a related strain in a captive colony of lesser dawn bats captured in Singapore. Genomic evidence of the virus was detected using targeted enrichment sequencing, and further investigated using deeper, unbiased high throughput sequencing. RoBat-CoV GCCDC1 Singapore shared 96.52% similarity with RoBat-CoV GCCDC1 356 (NC_030886) at the nucleotide level, and had a high prevalence in the captive bat colony. It was detected at five out of six sampling time points across the course of 18 months. A partial segment 1 from an ancestral Pteropine orthoreovirus, p10, makes up the recombinant portion of the virus, which shares high similarity with previously reported RoBat-CoV GCCDC1 strains that were detected in Yúnnan, China. RoBat-CoV GCCDC1 is an intriguing, cross-family recombinant virus, with a geographical range that expands farther than was previously known. The discovery of RoBat-CoV GCCDC1 in Singapore indicates that this recombinant coronavirus exists in a broad geographical range, and can persist in bat colonies long-term. View Full-Text
Keywords: lesser dawn bat; Rousettus bat coronavirus GCCDC1; recombinant; coronavirus lesser dawn bat; Rousettus bat coronavirus GCCDC1; recombinant; coronavirus
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Paskey, A.C.; Ng, J.H.J.; Rice, G.K.; Chia, W.N.; Philipson, C.W.; Foo, R.J.; Cer, R.Z.; Long, K.A.; Lueder, M.R.; Lim, X.F.; Frey, K.G.; Hamilton, T.; Anderson, D.E.; Laing, E.D.; Mendenhall, I.H.; Smith, G.J.; Wang, L.-F.; Bishop-Lilly, K.A. Detection of Recombinant Rousettus Bat Coronavirus GCCDC1 in Lesser Dawn Bats (Eonycteris spelaea) in Singapore. Viruses 2020, 12, 539.

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