Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mosquito Samples and Collections
2.2. RNA Extraction and Small RNA Deep Sequencing
2.3. Bioinformatics: Virus Discovery and RNAi Profile Analysis
2.4. Virus Genome Annotation and Phylogenetic Analysis
2.5. Construction and Validation of an AvIFV RT-PCR Screen for Ae. vexans spp. Mosquitoes
3. Results
3.1. Aedes vexans arabiensis Mosquitoes from Barkédji Village, Senegal, Were infected with a Novel Iflavirus
3.2. The Virus-Derived Small RNA Profile of Aedes Vexans Iflavirus
3.3. RT-PCR Screening of Ae. vexans spp. Samples from Senegal, Italy, the United Kingdom, Germany, and Sweden Suggests AvIFV is Exclusively Present in Ae. vexans Samples from Senegal
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Country | Location | Laboratory or Field | Coordinates | Date | Pools Screened | RT-PCR Result |
---|---|---|---|---|---|---|
SEN | Barkédji village | Laboratory | 15°17′ N, 14°53′ W | MAR 2020 | 10 | + |
SEN | Barkédji village | Laboratory | 15°17′ N, 14°53′ W | 2013 | 10 | + |
SEN | Barkédji village | Laboratory | 15°17′ N, 14°53′ W | 2013 | 10 | + |
SEN | Barkédji village | Field | 15°17′ N, 14°53′ W | 2011 | 10 | + |
GER | Großheide | Field | 53°36′ N, 7°21′ E | JUL 2017 | 1 | − |
GER | Varel | Field | 53°24 N, 8°8′ E | JUL 2017 | 1 | − |
GER | Rastede | Field | 53°14′ N, 8°12′ E | JUL 2017 | 1 | − |
GER | Oldenburg | Field | 53°6′ N, 8°15′ E | JUL 2017 | 1 | − |
GER | Bremen | Field | 53°5′ N, 8°51′ E | AUG 2017 | 1 | − |
GER | Hamburg | Field | 53°28′ N, 9°49′ E | AUG 2017 | 12 | − |
GER | Frankfurt a.M. | Field | 50°6′ N, 8°40′ E | AUG 2017 | 3 | − |
GER | Frankfurt a.M. | Field | 50°6′ N, 8°40′ E | SEP 2017 | 14 | − |
UK | Nottinghamshire | Field | 53°7′ N, 0°54′ W | JUL 2018 | 13 | − |
ITA | Guarda Veneta | Field | 44°58′ N, 11°47′ E | JUN 2019 | 1 | − |
ITA | Porto Tolle | Field | 44°54N, 12°27′ E | JUN 2019 | 1 | − |
ITA | Papozze | Field | 44°59′ N, 12°2′ E | JUN 2019 | 1 | − |
ITA | Ceggia | Field | 45°40′ N, 12°39′ E | JUN 2019 | 1 | − |
ITA | Riese Pio X | Field | 45°43′ N, 11°54′ E | JUN 2019 | 1 | − |
SWE | Forshaga | Field | 59°31′ N, 13°29′ E | JUN 2019 | 2 | − |
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Parry, R.; Naccache, F.; Ndiaye, E.H.; Fall, G.; Castelli, I.; Lühken, R.; Medlock, J.; Cull, B.; Hesson, J.C.; Montarsi, F.; et al. Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes. Viruses 2020, 12, 440. https://doi.org/10.3390/v12040440
Parry R, Naccache F, Ndiaye EH, Fall G, Castelli I, Lühken R, Medlock J, Cull B, Hesson JC, Montarsi F, et al. Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes. Viruses. 2020; 12(4):440. https://doi.org/10.3390/v12040440
Chicago/Turabian StyleParry, Rhys, Fanny Naccache, El Hadji Ndiaye, Gamou Fall, Ilaria Castelli, Renke Lühken, Jolyon Medlock, Benjamin Cull, Jenny C. Hesson, Fabrizio Montarsi, and et al. 2020. "Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes" Viruses 12, no. 4: 440. https://doi.org/10.3390/v12040440
APA StyleParry, R., Naccache, F., Ndiaye, E. H., Fall, G., Castelli, I., Lühken, R., Medlock, J., Cull, B., Hesson, J. C., Montarsi, F., Failloux, A.-B., Kohl, A., Schnettler, E., Diallo, M., Asgari, S., Dietrich, I., & Becker, S. C. (2020). Identification and RNAi Profile of a Novel Iflavirus Infecting Senegalese Aedes vexans arabiensis Mosquitoes. Viruses, 12(4), 440. https://doi.org/10.3390/v12040440