Proteome Analysis in a Mammalian Cell Line Reveals that PLK2 Is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis

Quan, Rong; Wei, Li; Hou, Lei; Wang, Jing; Zhu, Shanshan; Li, Zixuan; Lv, Moran; Liu, Jue

doi:10.3390/v12040375

Open AccessArticle

Proteome Analysis in a Mammalian Cell Line Reveals that PLK2 Is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis

by

Rong Quan

^†,

Li Wei

^†,

Lei Hou

,

Jing Wang

,

Shanshan Zhu

,

Zixuan Li

,

Moran Lv

and

Jue Liu

^*

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, No. 9 Shuguang Garden Middle Road, Haidian District, Beijing 100097, China

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Viruses 2020, 12(4), 375; https://doi.org/10.3390/v12040375

Submission received: 24 December 2019 / Revised: 25 March 2020 / Accepted: 26 March 2020 / Published: 28 March 2020

(This article belongs to the Section Animal Viruses)

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Abstract

Avian metapneumovirus subtype C (aMPV/C) causes an acute respiratory disease that has caused serious economic losses in the Chinese poultry industry. In the present study, we first explored the protein profile in aMPV/C-infected Vero cells using iTRAQ quantitative proteomics. A total of 921 of 7034 proteins were identified as significantly altered by aMPV/C infection. Three selected proteins were confirmed by Western blot analysis. Bioinformatics GO analysis revealed multiple signaling pathways involving cell cycle, endocytosis, and PI3K-Akt, mTOR, MAPK and p53 signaling pathways, which might participate in viral infection. In this analysis, we found that PLK2 expression was upregulated by aMPV/C infection and investigated whether it contributed to aMPV/C-mediated cellular dysfunction. Suppressing PLK2 attenuated aMPV/C-induced reactive oxygen species (ROS) production and p53-dependent apoptosis and reduced virus release. These results in a mammalian cell line suggest that high PLK2 expression correlates with aMPV/C-induced apoptosis and viral replication, providing new insight into the potential avian host cellular response to aMPV/C infection and antiviral targets.

Keywords: aMPV/C; differentially expressed proteins; iTRAQ; PLK2; apoptosis

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MDPI and ACS Style

Quan, R.; Wei, L.; Hou, L.; Wang, J.; Zhu, S.; Li, Z.; Lv, M.; Liu, J. Proteome Analysis in a Mammalian Cell Line Reveals that PLK2 Is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis. Viruses 2020, 12, 375. https://doi.org/10.3390/v12040375

AMA Style

Quan R, Wei L, Hou L, Wang J, Zhu S, Li Z, Lv M, Liu J. Proteome Analysis in a Mammalian Cell Line Reveals that PLK2 Is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis. Viruses. 2020; 12(4):375. https://doi.org/10.3390/v12040375

Chicago/Turabian Style

Quan, Rong, Li Wei, Lei Hou, Jing Wang, Shanshan Zhu, Zixuan Li, Moran Lv, and Jue Liu. 2020. "Proteome Analysis in a Mammalian Cell Line Reveals that PLK2 Is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis" Viruses 12, no. 4: 375. https://doi.org/10.3390/v12040375

APA Style

Quan, R., Wei, L., Hou, L., Wang, J., Zhu, S., Li, Z., Lv, M., & Liu, J. (2020). Proteome Analysis in a Mammalian Cell Line Reveals that PLK2 Is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis. Viruses, 12(4), 375. https://doi.org/10.3390/v12040375

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Proteome Analysis in a Mammalian Cell Line Reveals that PLK2 Is Involved in Avian Metapneumovirus Type C (aMPV/C)-Induced Apoptosis

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