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Cell Lines for Honey Bee Virus Research

1
Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611, USA
2
Biological Control of Insects Research Laboratory, USDA/Agricultural Research Service, Columbia, MO 65203, USA
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(2), 236; https://doi.org/10.3390/v12020236
Received: 15 January 2020 / Revised: 11 February 2020 / Accepted: 17 February 2020 / Published: 20 February 2020
(This article belongs to the Special Issue Advances in Honey Bee Virus Research)
With ongoing colony losses driven in part by the Varroa mite and the associated exacerbation of the virus load, there is an urgent need to protect honey bees (Apis mellifera) from fatal levels of virus infection and from the non-target effects of insecticides used in agricultural settings. A continuously replicating cell line derived from the honey bee would provide a valuable tool for the study of molecular mechanisms of virus–host interaction, for the screening of antiviral agents for potential use within the hive, and for the assessment of the risk of current and candidate insecticides to the honey bee. However, the establishment of a continuously replicating honey bee cell line has proved challenging. Here, we provide an overview of attempts to establish primary and continuously replicating hymenopteran cell lines, methods (including recent results) of establishing honey bee cell lines, challenges associated with the presence of latent viruses (especially Deformed wing virus) in established cell lines and methods to establish virus-free cell lines. We also describe the potential use of honey bee cell lines in conjunction with infectious clones of honey bee viruses for examination of fundamental virology. View Full-Text
Keywords: honey bee virus; Hymenoptera; insect cell culture; cell lines; Apis mellifera; deformed wing virus honey bee virus; Hymenoptera; insect cell culture; cell lines; Apis mellifera; deformed wing virus
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MDPI and ACS Style

Guo, Y.; Goodman, C.L.; Stanley, D.W.; Bonning, B.C. Cell Lines for Honey Bee Virus Research. Viruses 2020, 12, 236.

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