Significantly Improved Recovery of Recombinant Sonchus Yellow Net Rhabdovirus by Expressing the Negative-Strand Genomic RNA
Abstract
:1. Introduction
2. Materials and Methods
2.1. Construction of Plasmids
2.2. Agrobacterium Infiltration
2.3. Immunoblotting
2.4. Fluorescence Imaging
2.5. Transmission Electron Microscopy and Immunogold Labeling
3. Results
3.1. Use of Genome-Sense RNA Template Improves SYNV Rescue Efficiency by Two Orders of Magnitude
3.2. RNAi Suppression Is Essential for SYNV Recovery Using the gRNA Approach
3.3. Improved Rescue System Permits Recovery of a Highly Attenuated SYNV Mutant Virus
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Binary Plasmid a | OD600 | Foci No. b | Systemic Infection c |
---|---|---|---|
pSYNV-GFP(+) | 0.2 | 4.3 | 3/45 (6.7%) |
pSYNV-GFP(-) | 0.2 | >100 | 45/45 (100%) |
pSYNV-GFP(-) | 0.004 | 32.0 | 5/45 (11.1%) |
pSYNV-GFP(-) | 0.002 | 18.3 | 4/45 (8.8%) |
pSYNV-GFP(-) | 0.001 | 5.3 | 1/45 (2.2%) |
pSYNV-GFP-M:GFP(+) | 0.2 | 3.7 | 0/45 (0%) |
pSYNV-GFP-M:GFP(-) | 0.2 | 83.0 | 4/45 (8.8%) |
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Ma, X.; Li, Z. Significantly Improved Recovery of Recombinant Sonchus Yellow Net Rhabdovirus by Expressing the Negative-Strand Genomic RNA. Viruses 2020, 12, 1459. https://doi.org/10.3390/v12121459
Ma X, Li Z. Significantly Improved Recovery of Recombinant Sonchus Yellow Net Rhabdovirus by Expressing the Negative-Strand Genomic RNA. Viruses. 2020; 12(12):1459. https://doi.org/10.3390/v12121459
Chicago/Turabian StyleMa, Xiaonan, and Zhenghe Li. 2020. "Significantly Improved Recovery of Recombinant Sonchus Yellow Net Rhabdovirus by Expressing the Negative-Strand Genomic RNA" Viruses 12, no. 12: 1459. https://doi.org/10.3390/v12121459