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Article

Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures

1
Research Division, Federal Institute for Drugs and Medical Devices, 53175 Bonn, Germany
2
Department of Virology, Paul-Ehrlich-Institut, 63225 Langen, Germany
3
Institute of Reconstructive Neurobiology, LIFE & BRAIN Center, University of Bonn Medical Faculty & University Hospital Bonn, 53127 Bonn, Germany
4
Cell Programming Core Facility, Medical Faculty, University of Bonn, 53172 Bonn, Germany
5
Department of Clinical Pharmacology, University Hospital, RWTH Aachen University, 52074 Aachen, Germany
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(11), 1219; https://doi.org/10.3390/v12111219
Received: 9 September 2020 / Revised: 9 October 2020 / Accepted: 23 October 2020 / Published: 27 October 2020
(This article belongs to the Section Animal Viruses)
Zika virus (ZIKV) is a mosquito-borne virus, which can cause brain abnormalities in newborns, including microcephaly. MicroRNAs (miRNAs) are small non-coding RNAs, which post- transcriptionally regulate gene expression. They are involved in various processes including neurological development and host responses to viral infection, but their potential role in ZIKV pathogenesis remains poorly understood. MiRNAs can be incorporated into extracellular vesicles (EVs) and mediate cell-to-cell communication. While it is well known that in viral infections EVs carrying miRNAs can play a crucial role in disease pathogenesis, ZIKV effects on EV-delivered miRNAs and their contribution to ZIKV pathogenesis have not been elucidated. In the present study, we profiled intracellular and EV-derived miRNAs by next generation sequencing and analyzed the host mRNA transcriptome of neural stem cells during infection with ZIKV Uganda and French Polynesia strains. We identified numerous miRNAs, including miR-4792, which were dysregulated at the intracellular level and had altered levels in EVs during ZIKV infection. Integrated analyses of differentially expressed genes and miRNAs showed that ZIKV infection had an impact on processes associated with neurodevelopment and oxidative stress. Our results provide insights into the roles of intracellular and EV-associated host miRNAs in ZIKV pathogenesis. View Full-Text
Keywords: Zika virus; microRNA (miRNA); pathogenesis; extracellular vesicles; neural stem cells; flavivirus Zika virus; microRNA (miRNA); pathogenesis; extracellular vesicles; neural stem cells; flavivirus
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MDPI and ACS Style

Tabari, D.; Scholl, C.; Steffens, M.; Weickhardt, S.; Elgner, F.; Bender, D.; Herrlein, M.-L.; Sabino, C.; Semkova, V.; Peitz, M.; Till, A.; Brüstle, O.; Hildt, E.; Stingl, J. Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures. Viruses 2020, 12, 1219. https://doi.org/10.3390/v12111219

AMA Style

Tabari D, Scholl C, Steffens M, Weickhardt S, Elgner F, Bender D, Herrlein M-L, Sabino C, Semkova V, Peitz M, Till A, Brüstle O, Hildt E, Stingl J. Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures. Viruses. 2020; 12(11):1219. https://doi.org/10.3390/v12111219

Chicago/Turabian Style

Tabari, Denna, Catharina Scholl, Michael Steffens, Sandra Weickhardt, Fabian Elgner, Daniela Bender, Marie-Luise Herrlein, Catarina Sabino, Vesselina Semkova, Michael Peitz, Andreas Till, Oliver Brüstle, Eberhard Hildt, and Julia Stingl. 2020. "Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures" Viruses 12, no. 11: 1219. https://doi.org/10.3390/v12111219

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