Next Article in Journal
Recombinant Strains of Human Parechovirus in Rural Areas in the North of Brazil
Previous Article in Journal
Quantifying Anti-HIV Envelope-Specific Antibodies in Plasma from HIV Infected Individuals
Open AccessArticle

Isolation of a Divergent Strain of Bovine Parainfluenza Virus Type 3 (BPIV3) Infecting Cattle in China

1
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
Federal University of Pará, Belém 66075-000, Brazil
3
Beijing Observation Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing 100193, China
4
China Institute of Veterinary Drugs Control, Beijing 100083, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2019, 11(6), 489; https://doi.org/10.3390/v11060489
Received: 24 February 2019 / Revised: 18 April 2019 / Accepted: 23 April 2019 / Published: 29 May 2019
(This article belongs to the Section Animal Viruses)
Bovine parainfluenza virus type 3 (BPIV3) is one of the most important known viral respiratory pathogens of both young and adult cattle. It is also named “heat stress in transport”, causing morbidity and mass death. New variants of BPIV3 have been detected or isolated in China since 2008. Here, we isolate one BPIV3 strain (named BPIV3 BJ) in Madin-Darby bovine kidney (MDBK) cells from nasal samples collected in China. Phylogenetic analysis showed that our isolate is related to BPIV3 of the genotype A. The comparison of BPIV3-BJ and the reference Chinese isolate NM09 showed that these strains are highly divergent. We found many differences in the amino acid composition in the nucleocapsid (NP) protein among these genotype A strains. Since the NP protein has been implicated in immunization studies, our BPIV3 isolate will be useful for the development of immune assays and vaccine studies. The diversity of BPIV3 lineages that we found in China indicated ongoing evolution for immune escape. Our study highlights the importance of genetic surveillance for determining the effect of BPIV3 variability on pathogen evolution and population-scale immunity. View Full-Text
Keywords: bovine parainfluenza virus; vaccine; phylogenetic analysis; MDBK cells; cattle; biotechnology bovine parainfluenza virus; vaccine; phylogenetic analysis; MDBK cells; cattle; biotechnology
Show Figures

Figure 1

MDPI and ACS Style

Leal, É.; Liu, C.; Zhao, Z.; Deng, Y.; Villanova, F.; Liang, L.; Li, J.; Cui, S. Isolation of a Divergent Strain of Bovine Parainfluenza Virus Type 3 (BPIV3) Infecting Cattle in China. Viruses 2019, 11, 489.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop