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Open AccessArticle

Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum

by Feng Ding 1,2, Jiasen Cheng 1,2, Yanping Fu 2, Tao Chen 2, Bo Li 1,2, Daohong Jiang 1,2 and Jiatao Xie 1,2,*
1
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
2
The Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(3), 278; https://doi.org/10.3390/v11030278
Received: 22 February 2019 / Revised: 13 March 2019 / Accepted: 15 March 2019 / Published: 19 March 2019
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
We previously determined that virions of Sclerotinia sclerotiorum hypovirulence associated DNA virus 1 (SsHADV-1) could directly infect hyphae of Sclerotinia sclerotiorum, resulting in hypovirulence of the fungal host. However, the molecular mechanisms of SsHADV-1 virions disruption of the fungal cell wall barrier and entrance into the host cell are still unclear. To investigate the early response of S. sclerotiorum to SsHADV-1 infection, S. sclerotiorum hyphae were inoculated with purified SsHADV-1 virions. The pre- and post-infection hyphae were collected at one–three hours post-inoculation for transcriptome analysis. Further, bioinformatic analysis showed that differentially expressed genes (DEGs) regulated by SsHADV-1 infection were identified in S. sclerotiorum. In total, 187 genes were differentially expressed, consisting of more up-regulated (114) than down-regulated (73) genes. The identified DEGs were involved in several important pathways. Metabolic processes, biosynthesis of antibiotics, and secondary metabolites were the most affected categories in S. sclerotiorum upon SsHADV-1 infection. Cell structure analysis suggested that 26% of the total DEGs were related to membrane tissues. Furthermore, 10 and 27 DEGs were predicted to be located in the cell membrane and mitochondria, respectively. Gene ontology enrichment analyses of the DEGs were performed, followed by functional annotation of the genes. Interestingly, one third of the annotated functional DEGs could be involved in the Ras-small G protein signal transduction pathway. These results revealed that SsHADV-1 virions may be able to bind host membrane proteins and influence signal transduction through Ras-small G protein-coupled receptors during early infection, providing new insight towards the molecular mechanisms of virions infection in S. sclerotiorum. View Full-Text
Keywords: Sclerotinia sclerotiorum; Sclerotinia sclerotiorum hypovirulence associated DNA virus 1; mycovirus; transcriptome Sclerotinia sclerotiorum; Sclerotinia sclerotiorum hypovirulence associated DNA virus 1; mycovirus; transcriptome
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Ding, F.; Cheng, J.; Fu, Y.; Chen, T.; Li, B.; Jiang, D.; Xie, J. Early Transcriptional Response to DNA Virus Infection in Sclerotinia sclerotiorum. Viruses 2019, 11, 278.

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