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Viruses 2019, 11(3), 266; https://doi.org/10.3390/v11030266

Characterization of a Botybirnavirus Conferring Hypovirulence in the Phytopathogenic Fungus Botryosphaeria dothidea

1,2,†, 1,3,†, 2, 1,3 and 1,3,*
1
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430000, China
2
College of Life Science and Technology, Yangtze Normal University, Chongqing 400000, China
3
National Key Laboratory of Agromicrobiology, Huazhong Agricultural University, Wuhan 430000, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this study.
Received: 2 February 2019 / Revised: 12 March 2019 / Accepted: 14 March 2019 / Published: 17 March 2019
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Abstract

A double-stranded RNA (dsRNA) virus was isolated and characterized from strain EW220 of the phytopathogenic fungus Botryosphaeria dothidea. The full-length cDNAs of the dsRNAs were 6434 bp and 5986 bp in size, respectively. The largest dsRNA encodes a cap-pol fusion protein that contains a coat protein gene and an RNA-dependent RNA polymerase (RdRp) domain, and the second dsRNA encodes a hypothetical protein. Genome sequence analysis revealed that the sequences of the dsRNA virus shared 99% identity with Bipolaris maydis botybirnavirus 1(BmBRV1) isolated from the causal agent of corn southern leaf blight, Bipolaris maydis. Hence, the dsRNA virus constitutes a new strain of BmBRV1 and was named Bipolaris maydis botybirnavirus 1 strain BdEW220 (BmBRV1-BdEW220). BmBRV1-BdEW220 contains spherical virions that are 37 nm in diameter and consist of two dsRNA segments. The structural proteins of the BmBRV1-BdEW220 virus particles were 110 kDa, 90 kDa, and 80 kDa and were encoded by dsRNA1 and 2-ORFs. Phylogenetic reconstruction indicated that BmBRV1 and BmBRV1-BdEW220 are phylogenetically related to the genus Botybirnavirus. Importantly, BmBRV1-BdEW220 influences the growth of B. dothidea and confers hypovirulence to the fungal host. To our knowledge, this is the first report of a botybirnavirus in B. dothidea. View Full-Text
Keywords: Botryosphaeria dothidea; hypovirulence; Botybirnavirus; genome; double-stranded RNA virus Botryosphaeria dothidea; hypovirulence; Botybirnavirus; genome; double-stranded RNA virus
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    Description: Table S1. PMF-MS analysis of p110 encoded by ORF2 of Bipolaris maydis botybirnavirus 1 strain BdEW220; Table S2. PMF-MS analysis of p90 encoded by ORF1 of Bipolaris maydis botybirnavirus 1 strain BdEW220; Table S3. PMF-MS analysis of p80 encoded by ORF1 of Bipolaris maydis botybirnavirus 1 strain BdEW220; Figure S1. Multiple-sequence alignments of the dsRNA1 segment of BmBRV1-BdEW220, BmBRV1 and SsBRV1; Figure S2. Multiple-sequence alignments of the sequences of P1 encoded by BmBRV1-BdEW220, BmBRV1 and SsBRV1; Figure S3. Multiple-sequence alignments of the dsRNA2 segment of BmBRV1-BdEW220, BmBRV1 and SsBRV1; Figure S4. Multiple-sequence alignments of the sequences of P2 encoded by BmBRV1-BdEW220, BmBRV1 and SsBRV1; Figure S5. Genomic organization schematic diagrams of Botrytis porri botybirnavirus 1 (BpRV1), Bipolaris maydis botybirnavirus 1 strain BdEW220 (BmBRV1-BdEW220), Sclerotinia sclerotiorum botybirnavirus 1 (SsBRV1), and Alternaria botybirnavirus 1 (ABRV1).
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Zhai, L.; Yang, M.; Zhang, M.; Hong, N.; Wang, G. Characterization of a Botybirnavirus Conferring Hypovirulence in the Phytopathogenic Fungus Botryosphaeria dothidea. Viruses 2019, 11, 266.

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