Characterization of a Botybirnavirus Conferring Hypovirulence in the Phytopathogenic Fungus Botryosphaeria dothidea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Isolates and Culture Conditions
2.2. Extraction and Purification of dsRNA
2.3. cDNA Synthesis and Molecular Cloning
2.4. Sequence Analysis, Alignment, and Phylogenetic Analysis
2.5. Virion Purification
2.6. Vertical and Horizontal Transmission
2.7. Biological Testing
3. Results
3.1. Biological Characteristics of Strain EW220 and the Detection of dsRNA
3.2. Genetic Analysis of dsRNAs
3.3. Phylogenetic Analysis of the dsRNA Virus
3.4. Virus Particles
3.5. Structural Proteins of the Virus
3.6. Vertical and Horizontal Transmission of BmBRV1-BdEW220
3.7. Influence of BdBRV1 on the Biological Properties of B. dothidea
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Strain | Origin | BmBRV1-BdEW220 |
---|---|---|
EW220 | Pyrus pyrifolia, Wuhan, China | positive |
JNT1111 | P. bretschneideri, Shanxi, China | negative |
EW220-64 | A single-conidium isolate of EW220 | negative |
EW220-64-T1 | EW220-64 in a pairing culture of EW220-64 and EW220 | positive |
EW220-64-T2 | EW220-64 in a pairing culture of EW220-64 and EW220 | positive |
EW220-64-T3 | EW220-64 in a pairing culture of EW220-64 and EW220 | positive |
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Zhai, L.; Yang, M.; Zhang, M.; Hong, N.; Wang, G. Characterization of a Botybirnavirus Conferring Hypovirulence in the Phytopathogenic Fungus Botryosphaeria dothidea. Viruses 2019, 11, 266. https://doi.org/10.3390/v11030266
Zhai L, Yang M, Zhang M, Hong N, Wang G. Characterization of a Botybirnavirus Conferring Hypovirulence in the Phytopathogenic Fungus Botryosphaeria dothidea. Viruses. 2019; 11(3):266. https://doi.org/10.3390/v11030266
Chicago/Turabian StyleZhai, Lifeng, Mengmeng Yang, Meixin Zhang, Ni Hong, and Guoping Wang. 2019. "Characterization of a Botybirnavirus Conferring Hypovirulence in the Phytopathogenic Fungus Botryosphaeria dothidea" Viruses 11, no. 3: 266. https://doi.org/10.3390/v11030266