Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Antibodies and Reagents
2.3. Plasmid Construction
2.4. Infection and Treatment of PAMs
2.5. RNA Interference and Real-Time PCR
2.6. NLRP3 Inflammasome Reconstitution Assay
2.7. Co-Immunoprecipitation (Co-IP)
2.8. Porcine IL-1β ELISA
2.9. Western Blotting
2.10. Confocal Microscopy
2.11. Statistical Analysis
3. Results
3.1. SIV Infection Induces DRP1 Phosphorylation and Mitochondrial Fission
3.2. SIV-Induced Porcine IL-1β Production Is Dependent on ROS Production
3.3. SIV-Induced Porcine IL-1β Production Is Mediated by DRP1
3.4. RIPK1 Kinase Activity Is Critical for SIV-Induced IL-1β Production in PAMs
3.5. RIPK1 Interacts with DRP1
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Park, H.-S.; Liu, G.; Liu, Q.; Zhou, Y. Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production. Viruses 2018, 10, 419. https://doi.org/10.3390/v10080419
Park H-S, Liu G, Liu Q, Zhou Y. Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production. Viruses. 2018; 10(8):419. https://doi.org/10.3390/v10080419
Chicago/Turabian StylePark, Hong-Su, Guanqun Liu, Qiang Liu, and Yan Zhou. 2018. "Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production" Viruses 10, no. 8: 419. https://doi.org/10.3390/v10080419