Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production
Abstract
1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Antibodies and Reagents
2.3. Plasmid Construction
2.4. Infection and Treatment of PAMs
2.5. RNA Interference and Real-Time PCR
2.6. NLRP3 Inflammasome Reconstitution Assay
2.7. Co-Immunoprecipitation (Co-IP)
2.8. Porcine IL-1β ELISA
2.9. Western Blotting
2.10. Confocal Microscopy
2.11. Statistical Analysis
3. Results
3.1. SIV Infection Induces DRP1 Phosphorylation and Mitochondrial Fission
3.2. SIV-Induced Porcine IL-1β Production Is Dependent on ROS Production
3.3. SIV-Induced Porcine IL-1β Production Is Mediated by DRP1
3.4. RIPK1 Kinase Activity Is Critical for SIV-Induced IL-1β Production in PAMs
3.5. RIPK1 Interacts with DRP1
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Park, H.-S.; Liu, G.; Liu, Q.; Zhou, Y. Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production. Viruses 2018, 10, 419. https://doi.org/10.3390/v10080419
Park H-S, Liu G, Liu Q, Zhou Y. Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production. Viruses. 2018; 10(8):419. https://doi.org/10.3390/v10080419
Chicago/Turabian StylePark, Hong-Su, Guanqun Liu, Qiang Liu, and Yan Zhou. 2018. "Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production" Viruses 10, no. 8: 419. https://doi.org/10.3390/v10080419
APA StylePark, H.-S., Liu, G., Liu, Q., & Zhou, Y. (2018). Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production. Viruses, 10(8), 419. https://doi.org/10.3390/v10080419