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Viruses 2018, 10(8), 402; https://doi.org/10.3390/v10080402

Epigenetic Changes in the Regulation of Nicotiana tabacum Response to Cucumber Mosaic Virus Infection and Symptom Recovery through Single-Base Resolution Methylomes

1
College of Plant Protection, China Agricultural University, Beijing 100083, China
2
Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100083, China
3
College of Biological Sciences, China Agricultural University, Beijing 100083, China
4
Beijing Academy of Food Sciences, Beijing 100162, China
5
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
*
Author to whom correspondence should be addressed.
Received: 21 June 2018 / Revised: 20 July 2018 / Accepted: 27 July 2018 / Published: 29 July 2018
(This article belongs to the Special Issue Plant-Virus Interactions)
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Abstract

Plants have evolved multiple mechanisms to respond to viral infection. These responses have been studied in detail at the level of host immune response and antiviral RNA silencing (RNAi). However, the possibility of epigenetic reprogramming has not been thoroughly investigated. Here, we identified the role of DNA methylation during viral infection and performed reduced representation bisulfite sequencing (RRBS) on tissues of Cucumber mosaic virus (CMV)-infected Nicotiana tabacum at various developmental stages. Differential methylated regions are enriched with CHH sequence contexts, 80% of which are located on the gene body to regulate gene expression in a temporal style. The methylated genes depressed by methyltransferase inhibition largely overlapped with methylated genes in response to viral invasion. Activation in the argonaute protein and depression in methyl donor synthase revealed the important role of dynamic methylation changes in modulating viral clearance and resistance signaling. Methylation-expression relationships were found to be required for the immune response and cellular components are necessary for the proper defense response to infection and symptom recovery. View Full-Text
Keywords: Nicotiana tabacum; Cucumber mosaic virus; DNA methylation; gene expression; RNAi; demethylation Nicotiana tabacum; Cucumber mosaic virus; DNA methylation; gene expression; RNAi; demethylation
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Wang, C.; Wang, C.; Xu, W.; Zou, J.; Qiu, Y.; Kong, J.; Yang, Y.; Zhang, B.; Zhu, S. Epigenetic Changes in the Regulation of Nicotiana tabacum Response to Cucumber Mosaic Virus Infection and Symptom Recovery through Single-Base Resolution Methylomes. Viruses 2018, 10, 402.

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