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Viruses 2018, 10(6), 312; https://doi.org/10.3390/v10060312

Comparison of Porcine Airway and Intestinal Epithelial Cell Lines for the Susceptibility and Expression of Pattern Recognition Receptors upon Influenza Virus Infection

1
Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA
2
BioSNTR, Brookings, SD 57007, USA
3
Cambridge Technologies, Oxford Street Worthington, MN 56187, USA
4
Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA
*
Author to whom correspondence should be addressed.
Received: 5 March 2018 / Revised: 2 June 2018 / Accepted: 6 June 2018 / Published: 7 June 2018
(This article belongs to the Section Animal Viruses)
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Abstract

Influenza viruses infect the epithelial cells of the swine respiratory tract. Cell lines derived from the respiratory tract of pigs could serve as an excellent in vitro model for studying the pathogenesis of influenza viruses. In this study, we examined the replication of influenza viruses in the MK1-OSU cell line, which was clonally derived from pig airway epithelium. MK1-OSU cells expressed both cytokeratin and vimentin proteins and displayed several sugar moieties on the cell membrane. These cells also expressed both Sial2-3Gal and Sial2-6Gal receptors and were susceptible to swine influenza A, but not to human B and C viruses. Interestingly, these cells were also permissive to infection by influenza D virus that utilized 9-O-acetylated glycans. To study the differences in the expression of pattern recognition receptors (PRRs) upon influenza virus infection in the respiratory and digestive tract, we compared the protein expression of various PRRs in MK1-OSU cells with that in the SD-PJEC cell line, a clonally derived cell line from the porcine jejunal epithelium. Toll-like receptor 7 (TLR-7) and melanoma differentiation-associated protein 5 (MDA5) receptors showed decreased expression in influenza A infected MK1-OSU cells, while only TLR-7 expression decreased in SD-PJEC cells. Further research is warranted to study the mechanism behind the virus-mediated suppression of these proteins. Overall, this study shows that the porcine respiratory epithelial cell line, MK1-OSU, could serve as an in-vitro model for studying the pathogenesis and innate immune responses to porcine influenza viruses. View Full-Text
Keywords: MK1-OSU; SD-PJEC; respiratory epithelial cells; influenza; innate immunity; TLR; RIG-I-like receptor MK1-OSU; SD-PJEC; respiratory epithelial cells; influenza; innate immunity; TLR; RIG-I-like receptor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Thomas, M.; Pierson, M.; Uprety, T.; Zhu, L.; Ran, Z.; Sreenivasan, C.C.; Wang, D.; Hause, B.; Francis, D.H.; Li, F.; Kaushik, R.S. Comparison of Porcine Airway and Intestinal Epithelial Cell Lines for the Susceptibility and Expression of Pattern Recognition Receptors upon Influenza Virus Infection. Viruses 2018, 10, 312.

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