K15 Protein of Kaposi’s Sarcoma Herpesviruses Increases Endothelial Cell Proliferation and Migration through Store-Operated Calcium Entry
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Plasmids and Transfections
2.3. Production and Purification of Lentiviral Virions
2.4. Measurement of Cytosolic Ca2+
2.5. Western Blotting
2.6. Cell-Counting Kit-8 (CCK-8) Assay and Wound Scratch Assays
2.7. Statistical Analysis
3. Results
3.1. K15P Amplified Thapsigargin-Stimulated SOCE in HEK-293T and EA.hy926 Cells
3.2. Expression of STIM1 and Orail1in HEK-293T and EA.hy926 Cells
3.3. K15P Increased Cell Proliferation and Migration
4. Discussion
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Chen, W.; Xu, C.; Wang, L.; Shen, B.; Wang, L. K15 Protein of Kaposi’s Sarcoma Herpesviruses Increases Endothelial Cell Proliferation and Migration through Store-Operated Calcium Entry. Viruses 2018, 10, 282. https://doi.org/10.3390/v10060282
Chen W, Xu C, Wang L, Shen B, Wang L. K15 Protein of Kaposi’s Sarcoma Herpesviruses Increases Endothelial Cell Proliferation and Migration through Store-Operated Calcium Entry. Viruses. 2018; 10(6):282. https://doi.org/10.3390/v10060282
Chicago/Turabian StyleChen, Wei, Changqing Xu, Liuqing Wang, Bing Shen, and Linding Wang. 2018. "K15 Protein of Kaposi’s Sarcoma Herpesviruses Increases Endothelial Cell Proliferation and Migration through Store-Operated Calcium Entry" Viruses 10, no. 6: 282. https://doi.org/10.3390/v10060282