Zika Virus Induces Autophagy in Human Umbilical Vein Endothelial Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells
2.2. Antibodies and Chemicals
2.3. Plasmid Construction
2.4. Viral Infection
2.5. Lentiviral Particle Production and Target Cell Transduction
2.6. Plaque Assay
2.7. Confocal Microscopy
2.8. Western Blot Analysis
2.9. Statistical Analysis
3. Results
3.1. ZIKV Infection Induces Autophagy in HUVEC
3.2. Increased Levels of Autophagic Flux in ZIKV-Infected HUVEC
3.3. Pharmacological Inhibition of Autophagy Reduces ZIKV Production in HUVEC
3.4. Knockdown of Autophagy-Related Genes (ATGs) Reduces ZIKV Production in HUVEC
3.5. Rapamycin Does Not Promote ZIKV Production in HUVEC
4. Discussion
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Peng, H.; Liu, B.; Yves, T.D.; He, Y.; Wang, S.; Tang, H.; Ren, H.; Zhao, P.; Qi, Z.; Qin, Z. Zika Virus Induces Autophagy in Human Umbilical Vein Endothelial Cells. Viruses 2018, 10, 259. https://doi.org/10.3390/v10050259
Peng H, Liu B, Yves TD, He Y, Wang S, Tang H, Ren H, Zhao P, Qi Z, Qin Z. Zika Virus Induces Autophagy in Human Umbilical Vein Endothelial Cells. Viruses. 2018; 10(5):259. https://doi.org/10.3390/v10050259
Chicago/Turabian StylePeng, Haoran, Bin Liu, Toure Doueu Yves, Yanhua He, Shijie Wang, Hailin Tang, Hao Ren, Ping Zhao, Zhongtian Qi, and Zhaoling Qin. 2018. "Zika Virus Induces Autophagy in Human Umbilical Vein Endothelial Cells" Viruses 10, no. 5: 259. https://doi.org/10.3390/v10050259
APA StylePeng, H., Liu, B., Yves, T. D., He, Y., Wang, S., Tang, H., Ren, H., Zhao, P., Qi, Z., & Qin, Z. (2018). Zika Virus Induces Autophagy in Human Umbilical Vein Endothelial Cells. Viruses, 10(5), 259. https://doi.org/10.3390/v10050259