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Viruses 2018, 10(4), 194; https://doi.org/10.3390/v10040194

Phylogenetic Analysis and Pathogenicity Assessment of the Emerging Recombinant Subgroup K of Avian Leukosis Virus in South China

1,†
,
1,†
,
1,2,3,4,5
and
1,2,3,4,5,*
1
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
2
Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, Guangzhou 510642, China
3
South China Collaborative Innovation Center for Prevention and Control of Poultry Infectious Diseases and Safety of Poultry Products, Guangzhou 510642, China
4
National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangzhou 510642, China
5
Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 9 March 2018 / Revised: 7 April 2018 / Accepted: 10 April 2018 / Published: 13 April 2018
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Abstract

In recent years, cases of avian leukosis virus (ALV) infection have become more frequent in China. We isolated 6 ALV strains from yellow feather broiler breeders in south China from 2014 to 2016. Their full genomes were sequenced, compared, and analyzed with other reference strains of ALV. The complete genomic nucleotide sequences of GD150509, GD160403, GD160607, GDFX0601, and GDFX0602 were 7482 bp in length, whereas GDFX0603 was 7480 bp. They shared 99.7% to 99.8% identity with each other. Homology analysis showed that the gag, pol, long terminal repeats (LTRs), and the transmembrane region (gp37) of the env genes of the 6 viruses were well conserved to endogenous counterpart sequences (>97.8%). However, the gp85 genes displayed high variability with any known chicken ALV strains. Growth kinetics of DF-1 cells infected with the isolated ALV showed viral titers that were lower than those infected with the GD13 (ALV-A), CD08 (ALV-B), and CHN06 (ALV-J) on day 7 post-infection. The infected Specific-pathogen-free (SPF) chickens could produce continuous viremia, atrophy of immune organs, growth retardation and no tumors were observed. These subgroup ALVs are unique and may be common in south China. The results suggested that updating the control and eradication program of exogenous ALV for yellow feather broiler breeders in south China needs to be considered because of the emergence of the new subgroup viruses. View Full-Text
Keywords: avian leukosis virus subgroup K; complete genome; phylogenetic analysis; pathogenicity assessment avian leukosis virus subgroup K; complete genome; phylogenetic analysis; pathogenicity assessment
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Zhao, Z.; Rao, M.; Liao, M.; Cao, W. Phylogenetic Analysis and Pathogenicity Assessment of the Emerging Recombinant Subgroup K of Avian Leukosis Virus in South China. Viruses 2018, 10, 194.

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