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Viruses 2018, 10(4), 151; https://doi.org/10.3390/v10040151

Identification of a Novel Recombinant Type 2 Porcine Reproductive and Respiratory Syndrome Virus in China

1
School of Life Science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, 29# Wangjiang Road, Chengdu 610064, China
2
Sichuan Animal Science Academy, Sichuan Provincial Key laboratory of Animal Breeding and Genetics, Chengdu 610066, China
3
Chengdu Chia Tai Agro-Industry & Food Co., Ltd., Animal Healthy Disease Service, Gongping Town, Wenjiang District, Chengdu 610081, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 18 February 2018 / Revised: 20 March 2018 / Accepted: 22 March 2018 / Published: 27 March 2018
(This article belongs to the Section Animal Viruses)
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Abstract

Since the emergence of NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV) in China in 2013, PRRSVs have undergone rapid evolution. In this study, a novel variant of PRRSV strain (designated SCcd17) was successfully isolated from piglets with clinical signs in Sichuan Province in China in 2017, and the complete genomic sequence was determined. The genome of this new isolate was 15,015 nucleotides (nt) long, and comparative analysis revealed that SCcd17 exhibited 90.2%, 85.2%, 84.9%, and 84.0% nucleotide similarity to PRRSVs NADC30, JXA1, CH-1a, and VR-2332, respectively. Phylogenetic analysis indicated that the SCcd17 strain was classified into the NADC30-like sub-genotype, in which all the strains contained the unique discontinuous 131-amino acid deletion in nonstructural protein 2 (nsp2) when compared to VR-2332-like viruses. Notably, extensive amino acid substitutions were observed in nsp2 and a unique single amino acid deletion at position 33 of the GP5 is being described for the first time. Strikingly, recombination analysis revealed that SCcd17 was the result of recombination between the NADC30-like, JXA1-like, and VR-2332-like strains at five recombination breakpoints: nsp1α (nt 641), nsp3 (nt 5141), nsp10 (nt 9521), open reading frame 3 (ORF3) (nt 12,581), and ORF4 (nt 13,021). The genomic data of SCcd17 will be helpful for understanding the role of genomic recombination in the evolution of PRRSV. View Full-Text
Keywords: porcine reproductive and respiratory syndrome virus; sub-genotype; phylogenetic analysis; recombination porcine reproductive and respiratory syndrome virus; sub-genotype; phylogenetic analysis; recombination
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Zhou, L.; Kang, R.; Xie, B.; Tian, Y.; Wu, X.; Lv, X.; Yang, X.; Wang, H. Identification of a Novel Recombinant Type 2 Porcine Reproductive and Respiratory Syndrome Virus in China. Viruses 2018, 10, 151.

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