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Viruses 2018, 10(3), 125; https://doi.org/10.3390/v10030125

Human Parainfluenza Virus Type 3 Matrix Protein Reduces Viral RNA Synthesis of HPIV3 by Regulating Inclusion Body Formation

1,2,†
,
1,†
,
1,†
,
1,* and 1,*
1
State Key Laboratory of Virology and Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430000, China
2
Hubei University of Chinese Medicine, School of Laboratory Medicine, Wuhan 430000, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 13 January 2018 / Revised: 7 March 2018 / Accepted: 7 March 2018 / Published: 11 March 2018
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Abstract

Human parainfluenza virus type 3 is one of the main causes of lower respiratory illness in newborns and infants. The role of the matrix protein (M) in viral budding is extensively studied, but the effect of M on viral replication remains to be determined. Using an HPIV3 minigenome assay, we found that M reduced HPIV3 mingenome-encoded reporter activity even though it had an unspecific effect on the expression of cellular genes. Furthermore, the inhibition effect of M on viral RNA synthesis was proven to be independent of its virus-like particles (VLPs)’ release ability. A VLP’s defective mutant (ML302A) decreased the expression of minigenome reporter as wild type M did. Using an immunofluorescence assay, we found that M weakened the formation of inclusion bodies (IBs), although it did not co-localize with the IBs. Moreover, using another mutant, ML305A , which is defective in M-nucleoprotein (N) interaction, we found that ML305A had no effect on reporter activity and IB formation as the wild type of M did. Taken together, we conclude that M reduces the replication of HPIV3 and IB formation by M–N interaction. View Full-Text
Keywords: human parainfluenza virus type 3; viral replication; virus-like particles; inclusion body formation; M–N interaction human parainfluenza virus type 3; viral replication; virus-like particles; inclusion body formation; M–N interaction
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Zhang, S.; Cheng, Q.; Luo, C.; Qin, Y.; Chen, M. Human Parainfluenza Virus Type 3 Matrix Protein Reduces Viral RNA Synthesis of HPIV3 by Regulating Inclusion Body Formation. Viruses 2018, 10, 125.

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