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Viruses 2018, 10(2), 68;

High Whole-Genome Sequence Diversity of Human Papillomavirus Type 18 Isolates

National Institute for Public Health and the Environment (RIVM), Centre for Infectious Disease Control, 3721 MA Bilthoven, The Netherlands
Department of Pathology, Vrije Universiteit—University Medical Center (VUmc), 1081 HV Amsterdam, The Netherlands
Author to whom correspondence should be addressed.
Received: 24 January 2018 / Revised: 3 February 2018 / Accepted: 6 February 2018 / Published: 7 February 2018
(This article belongs to the Section Animal Viruses)
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Background: The most commonly found human papillomavirus (HPV) types in cervical cancer are HPV16 and HPV18. Genome variants of these types have been associated with differential carcinogenic potential. To date, only a handful of studies have described HPV18 whole genome sequencing results. Here we describe HPV18 variant diversity and conservation of persistent infections in a longitudinal retrospective cohort study. Methods: Cervical self-samples were obtained annually over four years and genotyped on the SPF10-DEIA-LiPA25 platform. Clearing and persistent HPV18 positive infections were selected, amplified in two overlapping fragments, and sequenced using 32 sequence primers. Results: Complete viral genomes were obtained from 25 participants with persistent and 26 participants with clearing HPV18 infections, resulting in 52 unique HPV18 genomes. Sublineage A3 was predominant in this population. The consensus viral genome was completely conserved over time in persistent infections, with one exception, where different HPV18 variants were identified in follow-up samples. Conclusions: This study identified a diverse set of HPV18 variants. In persistent infections, the consensus viral genome is conserved. The identification of only one HPV18 infection with different major variants in follow-up implies that this is a potentially rare event. This dataset adds 52 HPV18 genome variants to Genbank, more than doubling the currently available HPV18 information resource, and all but one variant are unique additions. View Full-Text
Keywords: HPV18; genetic epidemiology; whole genome sequencing HPV18; genetic epidemiology; whole genome sequencing

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Weele, P.; Meijer, C.J.; King, A.J. High Whole-Genome Sequence Diversity of Human Papillomavirus Type 18 Isolates. Viruses 2018, 10, 68.

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