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Article

Genetic Resistance to Avian Leukosis Viruses Induced by CRISPR/Cas9 Editing of Specific Receptor Genes in Chicken Cells

1
Institute of Molecular Genetics, Czech Academy of Sciences, Videnska 1083, CZ 14220 Prague 4, Czech Republic
2
BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, 254 49 Jílové u Prahy, Czech Republic
*
Author to whom correspondence should be addressed.
Current address: Max Planck Institute for Medical Research, Department of Biomolecular Mechanisms, Jahnstrasse 29, 69120 Heidelberg, Germany.
Viruses 2018, 10(11), 605; https://doi.org/10.3390/v10110605
Received: 12 October 2018 / Revised: 29 October 2018 / Accepted: 30 October 2018 / Published: 2 November 2018
Avian leukosis viruses (ALVs), which are pathogens of concern in domestic poultry, utilize specific receptor proteins for cell entry that are both necessary and sufficient for host susceptibility to a given ALV subgroup. This unequivocal relationship offers receptors as suitable targets of selection and biotechnological manipulation with the aim of obtaining virus-resistant poultry. This approach is further supported by the existence of natural knock-outs of receptor genes that segregate in inbred lines of chickens. We used CRISPR/Cas9 genome editing tools to introduce frame-shifting indel mutations into tva, tvc, and tvj loci encoding receptors for the A, C, and J ALV subgroups, respectively. For all three loci, the homozygous frame-shifting indels generating premature stop codons induced phenotypes which were fully resistant to the virus of respective subgroup. In the tvj locus, we also obtained in-frame deletions corroborating the importance of W38 and the four amino-acids preceding it. We demonstrate that CRISPR/Cas9-mediated knock-out or the fine editing of ALV receptor genes might be the first step in the development of virus-resistant chickens. View Full-Text
Keywords: avian leukosis virus; retrovirus receptor; virus-resistance in chicken; CRISPR/Cas9 avian leukosis virus; retrovirus receptor; virus-resistance in chicken; CRISPR/Cas9
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MDPI and ACS Style

Koslová, A.; Kučerová, D.; Reinišová, M.; Geryk, J.; Trefil, P.; Hejnar, J. Genetic Resistance to Avian Leukosis Viruses Induced by CRISPR/Cas9 Editing of Specific Receptor Genes in Chicken Cells. Viruses 2018, 10, 605. https://doi.org/10.3390/v10110605

AMA Style

Koslová A, Kučerová D, Reinišová M, Geryk J, Trefil P, Hejnar J. Genetic Resistance to Avian Leukosis Viruses Induced by CRISPR/Cas9 Editing of Specific Receptor Genes in Chicken Cells. Viruses. 2018; 10(11):605. https://doi.org/10.3390/v10110605

Chicago/Turabian Style

Koslová, Anna, Dana Kučerová, Markéta Reinišová, Josef Geryk, Pavel Trefil, and Jiří Hejnar. 2018. "Genetic Resistance to Avian Leukosis Viruses Induced by CRISPR/Cas9 Editing of Specific Receptor Genes in Chicken Cells" Viruses 10, no. 11: 605. https://doi.org/10.3390/v10110605

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