Phylogenetic Analysis and Characterization of a Sporadic Isolate of Equine Influenza A H3N8 from an Unvaccinated Horse in 2015
Abstract
:1. Introduction
2. Materials and Methods
2.1. Case Description
2.2. Whole Genome Sequencing and Phylogenetic Analysis
2.3. Virus and Cell Culture
2.4. Replication Kinetics
3. Results
3.1. BLAST Analysis
3.2. Phylogenetic Analysis
3.3. Viral Replication Kinetics
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Gene | Accession No. | Viruses with Highest % of Nucleotide Identity | Percent Identity |
---|---|---|---|
PB2 | MF599501.1 | A/equine/Malaysia/M201/2015 (H3N8) | 99.83 |
PB1 | KR351247.1 | A/equine/Tennessee/29A/2014 (H3N8) | 99.78 |
PA | KR351248.1 | A/equine/Tennessee/29A/2014 (H3N8) | 99.81 |
HA | KR351249.1 | A/equine/Tennessee/29A/2014 (H3N8) | 99.54 |
NP | KR351250.1 | A/equine/Tennessee/29A/2014 (H3N8) | 99.87 |
NA | KT867256.1 | A/equine/Malaysia/M201/2015 (H3N8) | 99.93 |
M | KR351252.1 | A/equine/Tennessee/29A/2014 (H3N8) | 99.8 |
NS | MF599503.1 | A/equine/Malaysia/M201/2015 (H3N8) | 100.0 |
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Sreenivasan, C.C.; Jandhyala, S.S.; Luo, S.; Hause, B.M.; Thomas, M.; Knudsen, D.E.B.; Leslie-Steen, P.; Clement, T.; Reedy, S.E.; Chambers, T.M.; et al. Phylogenetic Analysis and Characterization of a Sporadic Isolate of Equine Influenza A H3N8 from an Unvaccinated Horse in 2015. Viruses 2018, 10, 31. https://doi.org/10.3390/v10010031
Sreenivasan CC, Jandhyala SS, Luo S, Hause BM, Thomas M, Knudsen DEB, Leslie-Steen P, Clement T, Reedy SE, Chambers TM, et al. Phylogenetic Analysis and Characterization of a Sporadic Isolate of Equine Influenza A H3N8 from an Unvaccinated Horse in 2015. Viruses. 2018; 10(1):31. https://doi.org/10.3390/v10010031
Chicago/Turabian StyleSreenivasan, Chithra C., Sunayana S. Jandhyala, Sisi Luo, Ben M. Hause, Milton Thomas, David E. B. Knudsen, Pamela Leslie-Steen, Travis Clement, Stephanie E. Reedy, Thomas M. Chambers, and et al. 2018. "Phylogenetic Analysis and Characterization of a Sporadic Isolate of Equine Influenza A H3N8 from an Unvaccinated Horse in 2015" Viruses 10, no. 1: 31. https://doi.org/10.3390/v10010031
APA StyleSreenivasan, C. C., Jandhyala, S. S., Luo, S., Hause, B. M., Thomas, M., Knudsen, D. E. B., Leslie-Steen, P., Clement, T., Reedy, S. E., Chambers, T. M., Christopher-Hennings, J., Nelson, E., Wang, D., Kaushik, R. S., & Li, F. (2018). Phylogenetic Analysis and Characterization of a Sporadic Isolate of Equine Influenza A H3N8 from an Unvaccinated Horse in 2015. Viruses, 10(1), 31. https://doi.org/10.3390/v10010031