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Blood Coagulation Factor X Exerts Differential Effects on Adenovirus Entry into Human Lymphocytes

School of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, UK
Leeds Institute of Cancer and Pathology, University of Leeds, St. James’s University Hospital, Leeds LS9 7TF, UK
Author to whom correspondence should be addressed.
Viruses 2018, 10(1), 20;
Received: 5 December 2017 / Revised: 29 December 2017 / Accepted: 30 December 2017 / Published: 3 January 2018
(This article belongs to the Section Animal Viruses)
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It has been proposed that blood coagulation factors, principally factor X (FX), enhance the uptake of human adenovirus type 5 (Ad5) into cultured epithelial cells by bridging the viral hexon capsid protein and cell-surface heparan sulphate proteoglycans (HSPGs). We studied the effects of FX on Ad transduction of lymphoid cell lines (NK92MI, a natural killer cell line; Daudi, a B-cell line and Jurkat, a T-cell line) as well as primary peripheral blood lymphocytes (PBL) and HeLa epithelial cells using either replication-deficient Ad5, or a derivative in which the Ad5 fiber was replaced with that of another Ad type, Ad35, termed Ad5F35. PBL and NK92MI were resistant to Ad5 transduction. Transduction of Jurkat and Daudi cells by Ad5 was reduced by FX but without discernible effects on cell-surface Ad5 binding. FX reduced virus binding and transduction of all lymphoid cell lines by Ad5F35, as well as transduction of the T- and Natural Killer (NK)-cell populations of PBL. Flow cytometry analysis showed that all lymphoid cell lines were negative for HSPG components, in contrast to HeLa cells. FX reduced transduction of an HSPG-negative mutant Chinese hamster ovary cell line (CHOpgsA745) by Ad5 and Ad5F35, with Ad5F35 binding also being reduced by FX. These results point to fiber-dependent differences (Ad5 versus Ad35 fiber) in Ad binding to and transduction of human lymphoid and epithelial cells in the presence of FX. View Full-Text
Keywords: adenovirus; factor X; lymphoid cells; blood lymphocytes; virus entry adenovirus; factor X; lymphoid cells; blood lymphocytes; virus entry

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Findlay, J.S.; Cook, G.P.; Blair, G.E. Blood Coagulation Factor X Exerts Differential Effects on Adenovirus Entry into Human Lymphocytes. Viruses 2018, 10, 20.

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