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Molecular Characterization of Viruses from Clinical Respiratory Samples Producing Unidentified Cytopathic Effects in Cell Culture

Research Center in Infectious Diseases, CHUQ-CHUL and Laval University, Quebec City, QC, Canada
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Viruses 2009, 1(2), 84-90; https://doi.org/10.3390/v1020084
Received: 1 July 2009 / Revised: 14 July 2009 / Accepted: 16 July 2009 / Published: 17 July 2009
(This article belongs to the Special Issue Newly Identified Respiratory Viruses)
The sequence-independent single primer amplification (SISPA) method was performed to identify a virus in 17 clinical respiratory samples producing uncharacterized cytopathic effects in LLC-MK2 cells. Sequence analysis of 600-1600 bp amplicons allowed the identification of six viruses (one influenza C, two parechovirus-3 and three cardioviruses). Genomic sequences of the cardioviruses showed similarities with those of the recently-described Saffold virus strain although significant variation was present in the viral surface EF and CD loops. These results demonstrate the usefulness of SISPA for identifying emerging viruses and also known viruses not easily identified by standard virological methods. View Full-Text
Keywords: SISPA; respiratory viruses; picornaviruses; cardioviruses SISPA; respiratory viruses; picornaviruses; cardioviruses
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Abed, Y.; Boivin, G. Molecular Characterization of Viruses from Clinical Respiratory Samples Producing Unidentified Cytopathic Effects in Cell Culture. Viruses 2009, 1, 84-90.

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