Next Article in Journal
Estimating Individual Tree Height and Diameter at Breast Height (DBH) from Terrestrial Laser Scanning (TLS) Data at Plot Level
Previous Article in Journal
Stem Circadian Phenology of Four Pine Species in Naturally Contrasting Climates from Sky-Island Forests of the Western USA
Open AccessArticle

Transcriptome and Small RNA Sequencing Analysis Revealed Roles of PaWB-Related miRNAs and Genes in Paulownia fortunei

1
Institute of Paulownia, Henan Agricultural University, 95 Wenhua Road, Jinshui District, Zhengzhou 450002, China
2
Forestry Academy of Henan, Zhengzhou 450002, China
3
College of Forestry, Henan Agricultural University, 95 Wenhua Road, Jinshui District, Zhengzhou 450002, China
*
Author to whom correspondence should be addressed.
Forests 2018, 9(7), 397; https://doi.org/10.3390/f9070397
Received: 9 June 2018 / Revised: 29 June 2018 / Accepted: 3 July 2018 / Published: 4 July 2018
(This article belongs to the Section Forest Ecophysiology and Biology)
Paulownia witches’ broom (PaWB) is an epidemic disease caused by phytoplasmas infection, which is responsible for large production and economic losses. The study of PaWB has made significant progress, but the specific molecular mechanisms associated with PaWB remain unclear. To clearly know the gene expression profiles of plantlets infected with phytoplasmas, in this study, we used high-throughput sequencing technology to generate an integrated analysis of the transcriptome and microRNAs (miRNAs) of Paulownia fortunei (seem.) Hemsl. plantlets, and to obtain a comprehensive resource for the relationship between vital miRNA-target gene pairs and PaWB. A total of 756 genes, and 45 conserved and 22 new miRNAs were identified associated with PaWB. In addition, 635 target genes were predicted for the 67 DERs (Differentially expressed miRNAs). An interaction network of these miRNAs and their target genes was constructed. Gene ontology (GO) and KEGG pathway analysis of these target genes indicated that genes encoding transcription factors (TFs), including auxin response factors (ARF), WRKY, NAC (NAM, ATAF1/2 and CUC2), and MYB (v-myb avian myeloblastosis viral oncogene homolog), and genes encoding superoxide dismutase (SOD), as well as alternative splicing were related directly or indirectly to PaWB. Our results shed light on the possible roles of genes and miRNAs in PaWB-infected plantlets, which will enhance the understanding of the PaWB mechanism in Paulownia plants. View Full-Text
Keywords: Paulownia fortunei; paulownia witches’ broom; transcriptome; miRNAs; alternative splicing Paulownia fortunei; paulownia witches’ broom; transcriptome; miRNAs; alternative splicing
Show Figures

Graphical abstract

MDPI and ACS Style

Li, B.; Zhai, X.; Cao, Y.; Zhao, H.; Wang, Z.; Liu, H.; Fan, G. Transcriptome and Small RNA Sequencing Analysis Revealed Roles of PaWB-Related miRNAs and Genes in Paulownia fortunei. Forests 2018, 9, 397.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop