De Novo Sequencing and Assembly Analysis of Transcriptome in Pinus bungeana Zucc. ex Endl.
AbstractTo enrich the molecular data of Pinus bungeana Zucc. ex Endl. and study the regulating factors of different morphology controled by apical dominance. In this study, de novo assembly of transcriptome annotation was performed for two varieties of Pinus bungeana Zucc. ex Endl. that are obviously different in morphology. More than 147 million reads were produced, which were assembled into 88,092 unigenes. Based on a similarity search, 11,692 unigenes showed significant similarity to proteins from Picea sitchensis (Bong.) Carr. From this collection of unigenes, a large number of molecular markers were identified, including 2829 simple sequence repeats (SSRs). A total of 158 unigenes expressed differently between two varieties, including 98 up-regulated and 60 down-regulated unigenes. Furthermore, among the differently expressed genes (DEGs), five genes which may impact the plant morphology were further validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The five genes related to cytokinin oxidase/dehydrogenase (CKX), two-component response regulator ARR-A family (ARR-A), plant hormone signal transduction (AHP), and MADS-box transcription factors have a close relationship with apical dominance. This new dataset will be a useful resource for future genetic and genomic studies in Pinus bungeana Zucc. ex Endl. View Full-Text
- Supplementary File 1:
Supplementary (ZIP, 642 KB)
A printed edition of this Special Issue is available here.
Share & Cite This Article
Cai, Q.; Li, B.; Lin, F.; Huang, P.; Guo, W.; Zheng, Y. De Novo Sequencing and Assembly Analysis of Transcriptome in Pinus bungeana Zucc. ex Endl.. Forests 2018, 9, 156.
Cai Q, Li B, Lin F, Huang P, Guo W, Zheng Y. De Novo Sequencing and Assembly Analysis of Transcriptome in Pinus bungeana Zucc. ex Endl.. Forests. 2018; 9(3):156.Chicago/Turabian Style
Cai, Qifei; Li, Bin; Lin, Furong; Huang, Ping; Guo, Wenying; Zheng, Yongqi. 2018. "De Novo Sequencing and Assembly Analysis of Transcriptome in Pinus bungeana Zucc. ex Endl.." Forests 9, no. 3: 156.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.