Next Article in Journal
Shifts in Forest Structure in Northwest Montana from 1972 to 2015 Using the Landsat Archive from Multispectral Scanner to Operational Land Imager
Next Article in Special Issue
Tamarix microRNA Profiling Reveals New Insight into Salt Tolerance
Previous Article in Journal
Development of an Integrated DBH Estimation Model Based on Stand and Climatic Conditions
Previous Article in Special Issue
Use of Nuclear Microsatellite Loci for Evaluating Genetic Diversity of Selected Populations of Picea abies (L.) Karsten in the Czech Republic
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle

De Novo Sequencing and Assembly Analysis of Transcriptome in Pinus bungeana Zucc. ex Endl.

1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3 and 1,2,3,*
1
State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
2
Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
3
Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Chinese Academy of Forestry, Beijing 100091, China
*
Author to whom correspondence should be addressed.
Forests 2018, 9(3), 156; https://doi.org/10.3390/f9030156
Received: 24 January 2018 / Revised: 14 March 2018 / Accepted: 19 March 2018 / Published: 20 March 2018
(This article belongs to the Special Issue Genetics and Genomics of Forest Trees)
  |  
PDF [6179 KB, uploaded 3 May 2018]
  |  

Abstract

To enrich the molecular data of Pinus bungeana Zucc. ex Endl. and study the regulating factors of different morphology controled by apical dominance. In this study, de novo assembly of transcriptome annotation was performed for two varieties of Pinus bungeana Zucc. ex Endl. that are obviously different in morphology. More than 147 million reads were produced, which were assembled into 88,092 unigenes. Based on a similarity search, 11,692 unigenes showed significant similarity to proteins from Picea sitchensis (Bong.) Carr. From this collection of unigenes, a large number of molecular markers were identified, including 2829 simple sequence repeats (SSRs). A total of 158 unigenes expressed differently between two varieties, including 98 up-regulated and 60 down-regulated unigenes. Furthermore, among the differently expressed genes (DEGs), five genes which may impact the plant morphology were further validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The five genes related to cytokinin oxidase/dehydrogenase (CKX), two-component response regulator ARR-A family (ARR-A), plant hormone signal transduction (AHP), and MADS-box transcription factors have a close relationship with apical dominance. This new dataset will be a useful resource for future genetic and genomic studies in Pinus bungeana Zucc. ex Endl. View Full-Text
Keywords: De novo; Pinus bungeana Zucc. ex Endl.; transcriptome; assembly analysis De novo; Pinus bungeana Zucc. ex Endl.; transcriptome; assembly analysis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed
Printed Edition Available!
A printed edition of this Special Issue is available here.

Share & Cite This Article

MDPI and ACS Style

Cai, Q.; Li, B.; Lin, F.; Huang, P.; Guo, W.; Zheng, Y. De Novo Sequencing and Assembly Analysis of Transcriptome in Pinus bungeana Zucc. ex Endl.. Forests 2018, 9, 156.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Forests EISSN 1999-4907 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top