High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.
AbstractResearch Highlights: In the current study, we established a method for plant regeneration via somatic embryogenesis (SE) in Koelreuteria paniculata Laxm. for the first time. Background and Objectives: K. paniculata is an important ornamental and medicinal plant in China. However, the plant has difficulty with asexual reproduction, which imposes a limitation on large-scale propagation. Materials and Methods: Embryogenic calluses were induced from stems of aseptic seedlings on induction media. The effects of different media types and concentrations of N6-benzyladenine (BA), α-naphthaleneacetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D) on callus induction were examined. Embryogenic calluses were then transferred to Driver-Kuniyuki Walnut (DKW) media containing NAA (0.1–0.2 mg L−1) or 2,4-D (0.5–2.0 mg L−1) to develop somatic embryos. Cotyledon embryos were cultured on DKW media containing NAA (0.1–0.2 mg L−1) until maturation, and were then transferred to 1/2 DKW medium supplemented with 1.0 mg L−1 indole-3-butyric acid (IBA) to produce complete plants. The effects of IBA and NAA on rhizogenesis were then examined by clonal culture. Results: The maximum callus induction frequency (80.25%) was obtained on DKW medium supplemented by 0.5 mg L−1 BA, 0.25 mg L−1 NAA, and 1.5 mg L−1 2,4-D. NAA had a more pronounced effect on somatic embryo growth than did 2,4-D, with a maximum SE frequency (54.75%) observed with 0.1 mg L−1 NAA added to DKW medium. For clonal culture, the highest rooting rate (52%) was observed on 1/4 DKW medium containing 1.5 mg L−1 IBA. Histology studies confirmed the presence of embryogenic calluses and somatic embryos in different stages. Conclusions: This protocol provides a novel method for large-scale propagation of K. paniculata, and creates opportunities for genetic engineering in this species. View Full-Text
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Yang, X.; Yang, X.; Guo, T.; Gao, K.; Zhao, T.; Chen, Z.; An, X. High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.. Forests 2018, 9, 769.
Yang X, Yang X, Guo T, Gao K, Zhao T, Chen Z, An X. High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.. Forests. 2018; 9(12):769.Chicago/Turabian Style
Yang, Xiong; Yang, Xiaoyu; Guo, Ting; Gao, Kai; Zhao, Tianyun; Chen, Zhong; An, Xinmin. 2018. "High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.." Forests 9, no. 12: 769.
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