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Article

Single-Molecule Long-Read Sequencing of Zanthoxylum bungeanum Maxim. Transcriptome: Identification of Aroma-Related Genes

by 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2 and 1,2,*
1
College of Forestry, Northwest A&F University, Yangling, Xianyang 712100, China
2
Research Centre for Engineering and Technology of Zanthoxylum State Forestry Administration, Yangling, Xianyang 712100, China
*
Author to whom correspondence should be addressed.
Forests 2018, 9(12), 765; https://doi.org/10.3390/f9120765
Received: 12 November 2018 / Revised: 7 December 2018 / Accepted: 9 December 2018 / Published: 12 December 2018
(This article belongs to the Section Forest Ecophysiology and Biology)
Zanthoxylum bungeanum Maxim. is an economically important tree species that is resistant to drought and infertility, and has potential medicinal and edible value. However, comprehensive genomic data are not yet available for this species, limiting its potential utility for medicinal use, breeding programs, and cultivation. Transcriptome sequencing provides an effective approach to remedying this shortcoming. Herein, single-molecule long-read sequencing and next-generation sequencing approaches were used in parallel to obtain transcript isoform structure and gene functional information in Z. bungeanum. In total, 282,101 reads of inserts (ROIs) were identified, including 134,074 full-length non-chimeric reads, among which 65,711 open reading frames (ORFs), 50,135 simple sequence repeats (SSRs), and 1492 long non-coding RNAs (lncRNAs) were detected. Functional annotation revealed metabolic pathways related to aroma components and color characteristics in Z. bungeanum. Unexpectedly, 30 transcripts were annotated as genes involved in regulating the pathogenesis of breast and colorectal cancers. This work provides a comprehensive transcriptome resource for Z. bungeanum, and lays a foundation for the further investigation and utilization of Zanthoxylum resources. View Full-Text
Keywords: aroma metabolic pathway; long non-coding RNA; single-molecule real-time sequencing; Zanthoxylum bungeanum Maxim. aroma metabolic pathway; long non-coding RNA; single-molecule real-time sequencing; Zanthoxylum bungeanum Maxim.
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MDPI and ACS Style

Tian, J.; Feng, S.; Liu, Y.; Zhao, L.; Tian, L.; Hu, Y.; Yang, T.; Wei, A. Single-Molecule Long-Read Sequencing of Zanthoxylum bungeanum Maxim. Transcriptome: Identification of Aroma-Related Genes. Forests 2018, 9, 765. https://doi.org/10.3390/f9120765

AMA Style

Tian J, Feng S, Liu Y, Zhao L, Tian L, Hu Y, Yang T, Wei A. Single-Molecule Long-Read Sequencing of Zanthoxylum bungeanum Maxim. Transcriptome: Identification of Aroma-Related Genes. Forests. 2018; 9(12):765. https://doi.org/10.3390/f9120765

Chicago/Turabian Style

Tian, Jieyun, Shijing Feng, Yulin Liu, Lili Zhao, Lu Tian, Yang Hu, Tuxi Yang, and Anzhi Wei. 2018. "Single-Molecule Long-Read Sequencing of Zanthoxylum bungeanum Maxim. Transcriptome: Identification of Aroma-Related Genes" Forests 9, no. 12: 765. https://doi.org/10.3390/f9120765

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