Next Article in Journal
Full-Length Transcriptome-Wide Characteristic and Functional Identification of WRKY Family in Malus sieversii during the Valsa Canker Disease Response
Next Article in Special Issue
Metabolic and Transcriptional Profiling of Fraxinus chinensis var. rhynchophylla Unravels Possible Constitutive Resistance against Agrilus planipennis
Previous Article in Journal
Cellulose δ18O of Tree Rings Reflects Vapour Pressure Variations in the Ordos Plateau
Previous Article in Special Issue
Comparison and Phylogenetic Analyses of Nine Complete Chloroplast Genomes of Zingibereae
Article

Transcriptomic and Anatomic Profiling Reveal Etiolation Promotes Adventitious Rooting by Exogenous Application of 1-Naphthalene Acetic Acid in Robinia pseudoacacia L.

1
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Robinia pseudoacacia Engineering Technology Research Center of National Forestry and Grassland Administration, College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China
2
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
3
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China
4
College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
5
Cotton Research Institute Multan, Multan 60000, Pakistan
6
Department of Plant Breeding and Genetics, Ghazi University, Dera Ghazi Khan 32200, Pakistan
7
International Magnesium Institute, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this article.
Academic Editors: Tadeusz Malewski and Carol A. Loopstra
Forests 2021, 12(6), 789; https://doi.org/10.3390/f12060789
Received: 16 March 2021 / Revised: 3 June 2021 / Accepted: 10 June 2021 / Published: 15 June 2021
(This article belongs to the Special Issue Forest Genomics and Transcriptomics)
The process of etiolation contributes significantly to vegetative propagation and root formation of woody plants. However, the molecular interaction pattern of different factors for etiolated adventitious root development in woody plants remains unclear. In the present study, we explored the changes at different etiolation stages of adventitious root formation in Robinia pseudoacacia. Histological and transcriptomic analyses were performed for the etiolated lower portion of hypocotyls to ascertain the adventitious root responses. We found that the dark-treated hypocotyls formed roots earlier than the control. Exogenous application of NAA (0.3 mg/L) stimulated the expressions of about 310 genes. Among these, 155 were upregulated and 155 were downregulated. Moreover, differentially expressed genes (DEGs) were significantly enriched in multiple pathways, including the biosynthesis of secondary metabolites, metabolic pathway, plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis, and carbon metabolism. These pathways could play a significant role during adventitious root formation in etiolated hypocotyls. The findings of this study can provide novel insights and a foundation for further studies to elucidate the connection between etiolation and adventitious root formation in woody plants. View Full-Text
Keywords: histology; RNA-sequencing; in vitro; multiple pathways; auxin; expression profile; black locust; rooting stages; dark pretreatment histology; RNA-sequencing; in vitro; multiple pathways; auxin; expression profile; black locust; rooting stages; dark pretreatment
Show Figures

Figure 1

MDPI and ACS Style

Munir, M.Z.; Ud Din, S.; Imran, M.; Zhang, Z.; Pervaiz, T.; Han, C.; Un Nisa, Z.; Bakhsh, A.; Atif Muneer, M.; Sun, Y.; Li, Y. Transcriptomic and Anatomic Profiling Reveal Etiolation Promotes Adventitious Rooting by Exogenous Application of 1-Naphthalene Acetic Acid in Robinia pseudoacacia L. Forests 2021, 12, 789. https://doi.org/10.3390/f12060789

AMA Style

Munir MZ, Ud Din S, Imran M, Zhang Z, Pervaiz T, Han C, Un Nisa Z, Bakhsh A, Atif Muneer M, Sun Y, Li Y. Transcriptomic and Anatomic Profiling Reveal Etiolation Promotes Adventitious Rooting by Exogenous Application of 1-Naphthalene Acetic Acid in Robinia pseudoacacia L. Forests. 2021; 12(6):789. https://doi.org/10.3390/f12060789

Chicago/Turabian Style

Munir, Muhammad Z., Saleem Ud Din, Muhammad Imran, Zijie Zhang, Tariq Pervaiz, Chao Han, Zaib Un Nisa, Ali Bakhsh, Muhammad Atif Muneer, Yuhan Sun, and Yun Li. 2021. "Transcriptomic and Anatomic Profiling Reveal Etiolation Promotes Adventitious Rooting by Exogenous Application of 1-Naphthalene Acetic Acid in Robinia pseudoacacia L." Forests 12, no. 6: 789. https://doi.org/10.3390/f12060789

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop