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Article

Key Techniques for Somatic Embryogenesis and Plant Regeneration of Pinus koraiensis

1
State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin 150040, China
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Laboratory of Forest Genetics and Breeding, Institution of the Russian Academy of Sciences V.N., Sukachev Institute of Forest Siberian Branch of RAS, 660036 Krasnoyarsk, Russia
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Department of Cell Biology and Institute of Plant Physiology K.A., Timiryazev Russian Academy of Sciences, 127276 Moscow, Russia
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Department of Plant Physiology, Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia
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State Forestry and Grassland Administration Engineering Technology Research Center of Korean Pine, Harbin 150040, China
*
Authors to whom correspondence should be addressed.
Forests 2020, 11(9), 912; https://doi.org/10.3390/f11090912
Received: 23 June 2020 / Revised: 4 August 2020 / Accepted: 17 August 2020 / Published: 20 August 2020
(This article belongs to the Section Forest Ecophysiology and Biology)
Korean pine is the dominant species of Korean pine forests. It is an economically valuable species that yields oil, high-quality timber and nuts, and it offers great prospects for further development. Complete regenerated plants of Korean pine were obtained via somatic embryogenesis using megagametophytes as the explant. The seeds of 27 families of Korean pine were collected to induce embryogenic lines. We compared the effects of explant collection time, family and medium components (concentrations of sucrose, plant growth regulators and acid-hydrolyzed casein) on embryogenic lines induction. The effects of plant growth regulators and L-glutamine contents on the proliferation and maturation of embryogenic cell lines were studied, and the germinating ability of different cell lines was evaluated. The embryogenic lines induction percentage of Korean pine reached 33.33%. When 4.52 μmol·L−1 2,4-D and 2.2 μmol·L−1 6-BA were added to the medium of embryogenic lines proliferation, the ability of embryo maturation was the best (cell line 001#-100 was 135.71·g−1 fresh weight). Adding 1–1.5g L−1 L-glutamine to the proliferation medium can improve the ability of embryo maturation (cell line 001#-100 was 165.63·g−1 fresh weight). The germination percentage of the three cell lines tested was significant, and the highest was 66%. We report on successful regeneration and cryopreservation methods for somatic embryos of Korean pine. This technology could be used to propagate the excellent germplasm resources of Korean pine and to establish multi-varietal forestry. View Full-Text
Keywords: Korean pine; embryogenic lines; somatic embryos; regenerated plant; megagametophytes; cryopreservation Korean pine; embryogenic lines; somatic embryos; regenerated plant; megagametophytes; cryopreservation
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MDPI and ACS Style

Gao, F.; Peng, C.; Wang, H.; Tretyakova, I.N.; Nosov, A.M.; Shen, H.; Yang, L. Key Techniques for Somatic Embryogenesis and Plant Regeneration of Pinus koraiensis. Forests 2020, 11, 912. https://doi.org/10.3390/f11090912

AMA Style

Gao F, Peng C, Wang H, Tretyakova IN, Nosov AM, Shen H, Yang L. Key Techniques for Somatic Embryogenesis and Plant Regeneration of Pinus koraiensis. Forests. 2020; 11(9):912. https://doi.org/10.3390/f11090912

Chicago/Turabian Style

Gao, Fang, Chunxue Peng, Hao Wang, Iraida Nikolaevna Tretyakova, Alexander Mikhaylovich Nosov, Hailong Shen, and Ling Yang. 2020. "Key Techniques for Somatic Embryogenesis and Plant Regeneration of Pinus koraiensis" Forests 11, no. 9: 912. https://doi.org/10.3390/f11090912

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