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Comparative Transcriptomic Response of Two Pinus Species to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus

1
Centro de Biotecnologia Agrícola e Agro-alimentar do Alentejo (CEBAL)/Instituto Politécnico de Beja (IPBeja), 7801-908 Beja, Portugal
2
INIAV—Instituto Nacional de Investigação Agrária e Veterinária, 2780-157 Oeiras, Portugal
3
MED—Mediterranean Institute for Agriculture, Environment and Development, CEBAL—Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, 7801-908 Beja, Portugal
4
Faculdade de Ciências de Lisboa, BioISI, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal
5
Centro de Estudos Florestais, Instituto Superior de Agronomia, Universidade de Lisboa—Tapada da Ajuda, 1349-017 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Forests 2020, 11(2), 204; https://doi.org/10.3390/f11020204
Received: 10 January 2020 / Revised: 3 February 2020 / Accepted: 5 February 2020 / Published: 12 February 2020
(This article belongs to the Section Forest Ecology and Management)
Pine wilt disease (PWD) caused by pine wood nematode (PWN), Bursaphelenchus xylophilus, is a serious threat to global forest populations of conifers, in particular Pinus spp. Recently, the presence of PWN was reported in dead Yunnan pine (Pinus yunnanensis) trees under natural conditions. To further understand the potential impact caused by PWN in Yunnan pine populations, a transcriptional profiling analysis was performed over different time points (0 hours (h), 6 h, 24 h, 48 h, and 7 days) after PWN inoculation. A total of 9961 differentially expressed genes were identified after inoculation, which suggested a dynamic response against the pathogen, with a more intense pattern at 48 h after inoculation. The results also highlighted a set of biological mechanisms triggered after inoculation that provide valuable information regarding the response of Yunnan pine to PWN infection. When compared with maritime pine (Pinus pinaster), the Yunnan pine response was less complex and involved a smaller number of differentially expressed genes, which may be associated with the increased degree of resistance to PWN displayed by Yunnan pine. These results revealed different strategies to cope with PWN infection by these two pine species, which display contrasting degrees of susceptibility, especially in the timely perception of the infection and response magnitude.
Keywords: RNA-sequencing; transcriptome analysis; gene expression; pine wilt disease; Pinus pinaster; Bursaphelenchus xylophilus; Pinus yunnanensis RNA-sequencing; transcriptome analysis; gene expression; pine wilt disease; Pinus pinaster; Bursaphelenchus xylophilus; Pinus yunnanensis
MDPI and ACS Style

Gaspar, D.; Trindade, C.; Usié, A.; Meireles, B.; Fortes, A.M.; Guimarães, J.B.; Simões, F.; Costa, R.L.; Ramos, A.M. Comparative Transcriptomic Response of Two Pinus Species to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus. Forests 2020, 11, 204.

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