Morphological Characteristics and Transcriptome Comparisons of the Shoot Buds from Flowering and Non-Flowering Pleioblastus pygmaeus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Morphological and Anatomical Characterization
2.3. Sample and Library Preparation
2.4. Quality Control and Transcriptome Assembly
2.5. Gene Annotation and Differential Gene Expression
2.6. qRT-PCR Verification
3. Results
3.1. Morphological and Anatomical Characterization of the Samples
3.2. Data Filtering and Assembly
3.3. Gene Annotation
3.4. Differential Gene Enrichment Analysis
3.5. Expression Analysis of Key Genes Involved in Floral Development
3.6. RNA-Seq Expression Validation of Key Genes Involved in Floral Development
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
DEUs | differentially expressed unigenes |
TF | Transcription factor |
SEM | Scanning electron microscope |
GA | Gibberellins |
FE | Dormant shoot buds of flowering plants |
FM | Germinated shoots of flowering plants |
FL | Flower buds of flowering plants |
NE | Dormant shoot buds of vegetative plants |
NM | Germinated shoots of vegetative plants |
NL | Leaf buds of vegetative plants |
NR | NCBI non-redundant protein sequences |
NT | NCBI non-redundant nucleotide sequences |
Pfam | Protein family |
KOG | Eukaryotic ortholog group |
KEGG | Kyoto encyclopedia of genes and genomes |
GO | Gene ontology |
GC | guanine-cytosine |
bp | base pair |
RSEM | RNA-Seq by Expectation Maximization |
FPKM | Fragments per Kilobase Million |
TPM | Transcripts Perkilobase Million |
RRMs | RNA recognition motifs |
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Yao, W.; Li, C.; Lin, S.; Ren, L.; Wan, Y.; Zhang, L.; Ding, Y. Morphological Characteristics and Transcriptome Comparisons of the Shoot Buds from Flowering and Non-Flowering Pleioblastus pygmaeus. Forests 2020, 11, 1229. https://doi.org/10.3390/f11111229
Yao W, Li C, Lin S, Ren L, Wan Y, Zhang L, Ding Y. Morphological Characteristics and Transcriptome Comparisons of the Shoot Buds from Flowering and Non-Flowering Pleioblastus pygmaeus. Forests. 2020; 11(11):1229. https://doi.org/10.3390/f11111229
Chicago/Turabian StyleYao, Wenjing, Chuanzhe Li, Shuyan Lin, Li Ren, Yawen Wan, Li Zhang, and Yulong Ding. 2020. "Morphological Characteristics and Transcriptome Comparisons of the Shoot Buds from Flowering and Non-Flowering Pleioblastus pygmaeus" Forests 11, no. 11: 1229. https://doi.org/10.3390/f11111229