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Open AccessArticle

Transcriptomic Responses of Dove Tree (Davidia involucrata Baill.) to Heat Stress at the Seedling Stage

1
Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University, Nanchong 637009, China
2
Department of Plant Protection Biology, Swedish University of Agricultural Sciences, PO Box 102, SE-230 53 Alnarp, Sweden
3
College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010019, China
*
Author to whom correspondence should be addressed.
Forests 2019, 10(8), 656; https://doi.org/10.3390/f10080656
Received: 14 June 2019 / Revised: 30 July 2019 / Accepted: 1 August 2019 / Published: 5 August 2019
The dove tree (Davidia involucrata Baill.), a tertiary relic species, is adapted to cool climates. With the progression of global warming, high-temperature stress has become the primary environmental factor restricting geographic distribution, ex situ conservation, and landscape application for D. involucrata resources. However, the detailed molecular events underlying D. involucrata responses to heat stress are poorly understood. Here, we conducted RNA-Seq-based gene expression profiling in D. involucrata seedlings during the time course of a 42 °C heat treatment (0, 1, 6, and 12 h). After de novo assembly, we obtained 138,923 unigenes, of which 69,743 were annotated in public databases. Furthermore, 19,532, 20,497 and 27,716 differentially expressed genes (DEGs) were identified after 1 h (HS1), 6 h (HS6), and 12 h (HS12) of heat treatment in comparison to 0 h (HS0), respectively. Based on a KEGG enrichment analysis, the two pathways “protein processing in endoplasmic reticulum” and “plant hormone signal transduction” are hypothesized to play vital roles during heat response in D. involucrata, and their potential interactions during heat stress are also discussed. In addition, 32 genes encoding putative heat shock transcription factors (Hsfs) were found to be associated with the response of D. involucrata to heat stress. Finally, the expression patterns of eight heat-responsive genes derived from qRT-PCR were in agreement with their transcript level alterations, as determined by a transcriptome analysis. Taken together, our transcriptomic data provide the first comprehensive transcriptional profile affected by heat stress in D. involucrata, which will facilitate further studies on the improvement of heat tolerance in this rare and endangered species. View Full-Text
Keywords: Davidia involucrata Baill.; heat stress; transcriptome; RNA-Seq; DEG; Hsf Davidia involucrata Baill.; heat stress; transcriptome; RNA-Seq; DEG; Hsf
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Liu, Q.; Vetukuri, R.R.; Xu, W.; Xu, X. Transcriptomic Responses of Dove Tree (Davidia involucrata Baill.) to Heat Stress at the Seedling Stage. Forests 2019, 10, 656.

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