Next Article in Journal
Heterodichogamy, Pollen Viability, and Seed Set in a Population of Polyploidy Cyclocarya Paliurus (Batal) Iljinskaja (Juglandaceae)
Previous Article in Journal
Distribution and Habitat Selection of Free-Ranging European Bison (Bison bonasus L.) in a Mosaic Landscape—A Lithuanian Case
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Forests
Volume 10
Issue 4
10.3390/f10040346
forests-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Anthocyanin Synthesis and the Expression Patterns of bHLH Transcription Factor Family during Development of the Chinese Jujube Fruit (Ziziphus jujuba Mill.)

by
Qianqian Shi
1,2,
Xi Li
1,2,
Jiangtao Du
1,2 and
Xingang Li
1,2,3,*
1
College of Forestry, Northwest Agriculture and Forestry University, Yangling District, Xianyang 712100, China
2
Key Comprehensive Laboratory of Forestry of Shaanxi Province, Northwest A&F University, Yangling District, Xianyang 712100, China
3
Research Center for Jujube Engineering and Technology of State Forestry Administration, Northwest A&F University, Yangling District, Xianyang 712100, China
*
Author to whom correspondence should be addressed.
Forests 2019, 10(4), 346; https://doi.org/10.3390/f10040346
Submission received: 28 February 2019 / Revised: 10 April 2019 / Accepted: 17 April 2019 / Published: 19 April 2019
(This article belongs to the Section Forest Ecophysiology and Biology)
Browse Figures
Review Reports Versions Notes

Abstract

:
The basic helix–loop–helix (bHLH) family is an important transcription factor for eukaryotes and is involved in a wide range of biological activities. Among these, bHLH can interaction with WD repeat (WD40 or WDR) and V-myb avian myeloblastosis viral oncogene homolog (MYB) form a ternary complex to promote the efficient synthesis of anthocyanins. In this study, a total of 138 jujube bHLH (ZjbHLH) family members were screened from the transcriptome of the two jujube cultivars, ‘Junzao’ (JZ) and ‘Tailihong’ (TLH). Of these, 95 ZjbHLH genes were mapped to 12 chromosomes. A phylogenetic tree was constructed using 27 arabidopsis bHLH (AtbHLH) protein sequences of Arabidopsis thaliana (L.) Heynh. and 138 ZjbHLH protein sequences of jujube. The results show that the ZjbHLH family of jujube can be divided into 12 subfamilies. The three candidate genes, ZjGL3a, ZjGL3b and ZjTT8, related to anthocyanin synthesis, were classified into subgroup III. Meanwhile, ZjGL3a, ZjGL3b and ZjTT8 have high homology with the bHLH transcription factors involved in anthocyanin synthesis in other plants. In addition, it was found that the jujube ZjbHLH transcript family showed changing patterns of expression during fruit development. The relative expression levels of ZjGL3a, ZjGL3 and ZjTT8 were consistent with the changes of the anthocyanin contents in the two jujube cultivars examined. To better understand the anthocyanin synthesis pathway involved in ZjbHLH, a regulatory pathway model for anthocyanin synthesis was constructed. This model involves the processes of anthocyanin signal transduction, synthesis and transport.

1. Introduction

Chinese jujube (Ziziphus jujuba Mill.) belongs to the Rhamnaceae and is indigenous to China. It has been domesticated in China as a fruit crop for more than 7000 years [1]. This species is in high demand, due to its rich flavor and high nutritional value, in many Asian countries [2]. In more recent years, human nutritional science has demonstrated that jujube is a rich source of vitamins, flavonoids, polyphenols, fiber, sugars and other beneficial components [3]. Anthocyanins are widely distributed in the fruits, flowers, seeds and other tissues of the jujube plant. Here, they serve as antioxidants, attract insect pollinators, resist ultraviolet rays and participate in the plant’s responses to biotic and abiotic stresses [4]. The synthesis of anthocyanins is closely related to the flavonoid synthesis pathway. Sequence studies of different plant species have shown that the anthocyanin synthesis gene is strongly conserved, and also that the flavonoid synthesis gene is especially conservative [5].
The basic helix–loop–helix (bHLH) family of transcription factors (named for its basic-helix-loop-helix structure) is large and widely distributed in eukaryotes. Its core conserved domain contains approximately 60 amino acid residues [6]. The bHLH has been shown to be involved in numerous biological processes, including stress resistance [7,8,9], growth and development [10,11], signal transduction [12,13] and synthesis [14,15,16]. The first bHLH transcription factor was discovered in maize, where its function is involved in the synthesis of anthocyanins [17]. Other family members have subsequently been found in several species, including Arabidopsis [18], tobacco [19], rice [20] and tomato [21]. At this stage, 150 arabidopsis bHLH (AtbHLH) family members have been identified from Arabidopsis and have been shown to be involved in a range of processes, including the development of the reproductive system, transduction of phytochrome signaling, synthesis of secondary metabolites, and stress responses [22]. In addition, bHLH plays an important role in the synthesis of anthocyanins [23,24], and bHLH and myeloblastosis viral oncogene homolog (MYB) transcription factors can promote pigment synthesis in Arabidopsis seedlings [25]. In apples, the MdbHLH3 transcription factor has also been shown to promote anthocyanin accumulation in fruits [26]. The WD repeat (WD40) / basic helix–loop–helix (bHLH)/ V-myb avian myeloblastosis viral oncogene homolog (MYB) complex activates downstream signaling cascade under jasmonic acid (JA) induction, thereby regulating anthocyanin accumulation [27]. In addition, bHLH also plays an essential role in the synthesis of anthocyanins in gentian flowers [28].
In the color mutant jujube cultivar ‘Tailihong’ (TLH), the red color is due to anthocyanins [29]. Many studies have shown that bHLH transcription factors are involved in numerous physiological and biochemical processes in other plants. However, the molecular mechanisms of pigment formation in jujube fruit have not yet been elucidated, nor has the bHLH transcription factor family of jujube been fully identified. Therefore, our aim was to identify and analyze the bHLH transcription factors in the jujube genome and to investigate their changing expressions during fruit development. Our aim is that these findings will deepen our understanding of the functions of the jujube bHLH (ZjbHLH) genes in regulating the anthocyanin synthesis mechanism in jujube fruit.

2. Materials and Methods

2.1. Materials

Two jujube cultivars, Ziziphus jujuba Mill. ‘Junzao’ (JZ) and ‘Tailihong’ (TLH), were obtained from the Jujube Experimental Station of Northwest Agriculture and Forestry University in Qingjian, Shaanxi, China. A total of 20 fruit samples of each genotype from three jujube trees were harvested at six developmental stages (S1…S6) on days 30, 50, 80, 90, 100 and 110 after anthesis (DAA), respectively (see Figure S1). The fruit skins (about 2 mm thick) were removed with a domestic vegetable peeler randomly. Composite skin samples were immediately frozen in liquid nitrogen and held at −80 °C pending analysis.

2.2. Extraction and Analyses of Total Anthocyanins

Samples (about 0.5 g) of jujube fruit skin were extracted with 5 mL of 0.1% HCl in methanol for 24 h, at 4 °C in the dark. After centrifuging at 12,000 rpm for 15 min, three replicates were used for each sample [29]. The total anthocyanin content (TAC) was measured by the pH differential method. Absorbance was measured at 510 and 700 nm in buffers at pH 1.0 and at 4.5. The TAC is expressed as cyanidin-3-glucoside equivalents.

2.3. Total RNA Extraction and cDNA Synthesis

The total RNA of all samples was extracted using the TaKaRa MiniBEST Plant RNA Extraction Kit (TaKaRa, Beijing, China) according to the manufacturer’s instructions. The purity and concentration of the extracted total RNA were determined using NanoDrop 20000 (Thermo Scientific, Pittsburgh, PA, USA). Samples with an OD260/280 ratio of 1.8 to 2.0 and an OD260/230 ratio greater than 2.0 were retained. Subsequently, total RNA was reverse transcribed to obtain first strand cDNA.

2.4. Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) Analysis

Primers for real-time Quantitative PCR (RT-qPCR) were designed using Primer Premier 7.0 software (Premier, Palo Alto, CA, USA) (Table 1). The relative expression levels of target genes were detected using total RNA from different developmental stages of the two jujube cultivars as templates in CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). ZjUBQ and ZjUBQ2 were used as reference genes to correct the data [30]. The reaction system was 10 μL, including 1 μL cDNA, 1 μL each of the upstream and downstream primers, 5 μL of 2 × SYBR Premix Ex Taq II (TaKaRa) and 2 μL of ddH2O. The PCR reaction procedure was 95 °C for 10 s, 58 °C for 30 s, 72 °C for 45 s, 35 cycles of the above three steps and 72 °C for 5 min to complete the reaction.

2.5. Sequence Bioinformatics Analysis

Phylogenetic tree analysis of the bHLH transcription factor family obtained was generated using MEGA 7.0 (Center for Evolutionary Medicine and Informatics, Tempe, AZ, USA). The conserved domain of the bHLH transcription factor family protein sequence was analyzed using DNAMAN 8.0 (Lynnon Biosoft, San Ramon, CA, USA) and the online website WebLogo 3 (http://weblogo.berkeley.edu/logo.cgi). At the same time, their motif domains were predicted through the meme-suite website (http://meme-suite.org/). Chromosome localization of jujube ZjbHLH transcription factors was carried out using MapInspect 1.0 software (https://mapinspect.software.informer.com/1.0/). Analysis of the tertiary structure of the anthocyanin synthesis-related transcription factor protein was carried out using the online website, phyre2 (http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgibid=index).

2.6. Analysis of ZjbHLH Gene Expression from RNA-Seq Data

RNA-Seq reads were obtained with an Illumina HiSeq 2000 (Illumina, San Diego, CA, USA). The fragments per kilobase of exon per million mapped reads (FPKM) values were calculated based on RNA-Seq reads. The heatmap was generated with TBTOOLS software (https://github.com/CJ-Chen/TBtools) [31]. The color scale shown represents FPKM counts, and the ratios were log2 transformed.

3. Results

3.1. Analysis of Biological Information of the Jujube bHLH Transcription Factor Family

A total of 145 ZjbHLH transcriptome sequences were screened from the JZ genome and the sequence was analyzed by the Pfam online site (http://pfam.xfam.org/) to remove the incomplete sequence of the conserved domain. Finally, 138 ZjbHLH transcription factor family members were obtained. The amino acid sequences of the bHLH transcription factors of jujube and Arabidopsis thaliana were analyzed and classified using the MEGA 7.0 software with the bootstrap values from 1000 replicates. The ZjbHLH genes were divided into 12 subfamilies (Figure 1A) [32]. Among these, Group Ⅱ and Group Ⅵ members contained only two ZjbHLH transcription factors, while Group Ⅴ contains 30 ZjbHLH members. Three candidate ZjbHLH transcription factors (TFs) were found to belong to Group Ⅲ and are highly correlated with anthocyanin synthesis (ZjGL3a, ZjGL3b and ZjTT8). To provide additional support for this selection, phylogenetic analyses indicated that ZjTT8, ZjGL3a and ZjGL3b belong to the anthocyanin identified in other plants (Figure 1B). The protein sequences of ZjTT8 are most closely related to MabHLH3 and FabHLH3, sharing 78% and 71% amino acid identity, respectively. ZjGL3b showed the highest amino acid sequence identity with PhJAF13 (50%), while ZjGL3a showed the highest amino acid sequence identity with MabHLH33 (67%). MdbHLH3, PhJAF13 and MdbHLH3 have been shown to interact physically with MYB TFs to regulate several structural genes involved in the synthesis of anthocyanin [4,26,33]. Figure 1C shows the gene IDs and physiological and biochemical characteristics of the three candidate genes (ZjTT8, ZjGL3a and ZjGL3b). The lengths of the protein sequences of ZjTT8, ZjGL3a and ZjGL3b are 642, 637 and 705 amino acids, respectively. Their molecular weights (Mw) are 72694.97, 70922.78 and 78688.7 Da, respectively. Their isoelectric point (pI) values are 5.63, 5.19 and 5.22, respectively. Their aliphatic index (Ai) is 79.89, 84.65 and 74.16, respectively. The grand average values of hydropathicity (GRAVY) of the candidate ZjbHLHs were −0.545, −0.478 and −0.632, respectively.
The chromosomal distribution map of jujube bHLH genes was generated on the basis of jujube genome data (Figure 2). The ZjbHLH transcription factor family was mapped to the 12 chromosomes of jujube (2n = 2X = 24). The distribution of ZjbHLH transcription factors on the chromosomes is not uniform - there are 13 members on chromosomes one and six but only three on chromosome two. The three candidate genes (ZjTT8, ZjGL3a and ZjGL3b) was found on chromosomes 11, chromosomes 12 and chromosomes, respectively.
The ZjbHLH family protein conserved domain of jujube has a typical Basic-Helix-Loop-Helix structure (Figure 3) [34]. The ZjbHLH domain consists of about 60 amino acids, which contain two different functional regions, the C-terminal HLH region and the N-terminal basic amino acid region consisting of 10–15 amino acids. Interestingly, all members had a typical H5-E9-RR13 sequence (His5-Glu9-Arg13) and a few contained a R8-E9 sequence, which were required for specific binding of the target ZjbHLH protein to the target DNA. According to the meme-suite website (http://meme-suite.org/), all the bHLH members were subjected to motif analysis. Five motif structures were found (see Figures S2 and S3).

3.2. ZjbHLH Transcription Factor Family Expression Pattern by RNA-Seq Data

To further confirm the function of ZjbHLHs during development and ripening processes in jujube fruit, we explored the expression patterns of each gene using RNA-Seq. As shown in Table S1, a total of 51,073,436–64,668,776 raw reads were generated from the eighteen libraries. After filtration, a total of 49,129,642-62,254,438 clean reads were obtained from the eighteen libraries with an average Raw Q20 and Q30 base rate of nearly 95% and 90%, respectively.
The expression patterns of all ZjbHLH transcription factor families of fruit development in the two jujube cultivars, JZ and TLH, were analyzed (Figure 4). We found 71 ZjbHLH significantly expressed transcription factors. A heat map was constructed from these 71 significantly expressed transcription factors. At the same time, based on the expression trend, the ZjbHLH transcription factors were divided into four groups and the expression pattern of ZjbHLH transcription factors was similar in each group. From the heat map, we find that the various members have distinct expression patterns during fruit development. Also, ZjbHLH expressions differed between the two cultivars. Interestingly, the expression levels of most ZjbHLH transcription factors decreased during maturation. In addition, ZjGL3a, ZjGL3b and ZjTT8, associated with anthocyanin synthesis, have similar expression patterns and are clustered in the same branch.

3.3. Expression of ZjGL3a, ZjGL3b and ZjTT8 Correlated with Anthocyanin Content

To determine whether the changing expressions of ZjGL3a, ZjGL3b and ZjTT8 in jujube fruit correlate with changes in anthocyanin content during development, fruit of JZ and TLH were collected for analysis by the pH differential method and real-time quantitative PCR (Figure 5). In S1, the anthocyanin content in TLH was significantly higher than in JZ. During fruit development and ripening, the anthocyanin contents of both cultivars decreased rapidly. The anthocyanin content of TLH in S3 decreased strongly to 12% of that at S1, while the anthocyanin content in JZ was not detectable in S3. The sequence homology of the data ZjGL3a, ZjGL3b and ZjbTT8 with grape and orange was high by evolutionary analysis and their expression levels were consistent with the trend for anthocyanin content. Hence, we questioned if ZjGL3a, ZjGL3b and ZjbTT8 might be involved in the regulation of anthocyanin synthesis and if they might also be key regulators of anthocyanin synthesis in jujube. In other fruit species, such as apple and sweet cherry, the expressions of the anthocyanin regulators MdbHLH3 [26] and PabHLH3 [35] are closely correlated with gene expression.

4. Discussion

Jujube is an important economic species of the Rhamnaceae family. The fruit of jujube is rich in nutrients, which can provide essential amino acids and have strong antioxidant activity. Studying the transcription factor family of jujube is great significance for breeding excellent cultivars and improving fruit quality. However, until now, this TF family has not been reported in jujube. The bHLH transcription factor family has been identified in many species, for example, tomato [21], peach [36], apple [37] and potato [38]. They have many members, various functions, and plays an important role in the growth, metabolism, biosynthesis and stress tolerance of organisms [39,40,41].
The cluster analysis identified 138 bHLH transcription factors in jujube, which were divided into 12 subfamilies (Figure 1A) based on the reported evolutionary relationships Arabidopsis. The III subfamily of bHLH transcription factors play an irreplaceable role on the regulation of fruit anthocyanins synthesis [24,26]. Interestingly, ZjGL3a, ZjGL3b and ZjTT8 were classified into the III subfamily. The ZjbHLH domain consists of about 60 amino acids, which contain two different functional regions, the C-terminal HLH region and the N-terminal basic amino acid region consisting of 10–15 amino acids. And all members had a typical H5-E9-R13 sequence (His5-Glu9-Arg13) and a few contained a R8–E9 sequence, which were required for specific binding of the target ZjbHLH protein to the target DNA [42]. Analyzed the conserved domain of the ZjbHLH protein sequence showed that the most common amino acids at positions 5, 9 and 13 were histidine, glutamate and arginine, respectively. The analysis of ZjbHLH gene expression indicated that the expression levels of ZjGL3a, ZjGL3b, and ZjTT8 were consistent with the trend of jujube anthocyanin content. Therefore, ZjGL3a, ZjGL3b and ZjTT8 play an important role in anthocyanin synthesis.
The MBW complex formed by the combination of MYB, bHLH and WD40 plays a key role in inducing the anthocyanin synthesis pathway (Figure 6). The MBW complex specifically recognizes the MYB- recognition elements (MRE) and the bHLH- recognition elements (BRE). The transcription of the target gene is promoted by binding to a recognition site, upstream of the anthocyanin synthesis-related gene. The synthesis of anthocyanins is a branch of flavonoid synthesis and the synthesis of both substances starts from phenylalanine. Then coumaroyl-Co-A is synthesized from phenylalanine. Coumaroyl-Co-A synthesized chalcones under the action of chalcone synthase (CHS). Chalcones form flavanones under the catalysis of chalcone isomerase (CHI) and flavanones in flavanone 3-hydroxylase dihydroflavonols are formed by the action of (F3H) and flavonoid 3’-hydroxylase (F3’H), and leucoanthocyanidins are formed by dihydroflavonols under the action of dihydroflavonol 4-reductase (DFR). Subsequently, leucoanthocyanidins form anthocyanidins under the catalysis of anthocyanidin synthase (ANS). Finally, anthocyanidins form anthocyanins under the action of UDP g1ucose-flavonoid 3-O-glucosyltransferase (UFGT). The MBW complex is involved in multiple reaction steps of anthocyanin synthesis and is capable of binding to CHI, F3H, F3’H, DFR, ANS and UFGT and promoting the synthesis of anthocyanins [43]. ZjGL3a, ZjGL3b and ZjTT8 have high homology with bHLH transcription factors involved in anthocyanin synthesis in other fruit species, revealing that ZjGL3a, ZjGL3b and ZjTT8 may play an important role in anthocyanin synthesis of jujube fruits. In addition, bHLH is also used as a messenger of the external signal and can interact with WD40 and MYB to form a complex to promote the efficient synthesis of anthocyanins. There is a close interaction between environment and plant growth, with plants activating or inhibiting various signaling pathways in response to environmental change. Thus, light can activate the MYB transcription factor involved in anthocyanin synthesis, thereby promoting the accumulation of anthocyanins [44,45]. Water deficit during fruit development can enhance the regulation of anthocyanin synthesis by various signaling pathways. The main route may be that plants synthesize ABA activates ABA signaling pathway under drought stress and then promotes anthocyanin synthesis. The expressions of related genes promote fruit ripening and the accumulation of anthocyanins [46,47]. Hormones [27], sugar [48,49,50], organic acids [51] have also been widely involved in the synthesis, modification and transport of anthocyanins. Hormones, sucrose and organic acids promote the expressions of anthocyanin-related genes by activating the MYB transcription factor family and forming complexes with bHLH and WDR. At the same time, some MYB members are also able to directly regulate the synthesis of related proteins, but the promotion effect is not as strong as the complex.
In general, anthocyanins are synthesized on the Golgi bodies, some are oxidatively denatured and most are eventually transported to the vacuole for storage. There are three general ways to transport anthocyanins into the vacuole [52,53,54]. (1) Anthocyanin is transported to the vicinity of the vacuole with the help of GST, and then recognized by MRP on the tonoplast and transported into the vacuole; (2) The MATE transmembrane transporter on the vacuolar membrane can be directly transported into the vacuole, which depends on the H+ concentration gradient generated by the H+-ATPase proton pump; (3) Anthocyanins can be directly encapsulated into the vacuole by vesicles.

5. Conclusions

We identified and classified 138 ZjbHLH transcription factor family members in the jujube genome. Based on genetic analysis, the jujube bHLH transcription factors were divided into 12 subfamilies and found to be located on 12 chromosomes. The ZjGL3a, ZjGL3b and ZjTT8 transcription factors associated with anthocyanin synthesis are all classified in group III. ZjGL3a, ZjGL3b and ZjTT8 are highly homologous to the anthocyanin synthesis genes in grape and orange, and their expression levels are consistent with anthocyanin content. This shows they are important transcription factors involved in anthocyanin regulation in jujube fruits. The expression patterns of bHLH transcription factors in the jujube cultivars TLH and JZ were examined during development. It was found the expression levels of most transcription factors showed a downward trend. The content of total anthocyanins in TLH and JZ gradually decreased during fruit development. The relative expression levels of ZjGL3a, ZjGL3b and ZjTT8 transcription factors associated with anthocyanin synthesis also showed a downward trend. An anthocyanin synthesis-transport model centered on the WDR/bHLH/MYB complex was established to provide a reference for further understanding of the regulation of anthocyanin synthesis.

Supplementary Materials

The following are available online at https://www.mdpi.com/1999-4907/10/4/346/s1, Figure S1: The pictures of two jujube fruit developments. Figure S2: Jujube ZjbHLH transcription factor protein motif structure. Figure S3: Conserved motif analysis of Jujube of ZjbHLH gene family. Table S1: Statistic Reads of RNA-Seq data.

Author Contributions

Q.S. designed the experiments, wrote and revised the manuscript. X.L. (Xi Li) and J.D. helped revised manuscript. X.L. (Xingang Li) designed the experiments, discussed results, and revised the manuscript.

Funding

This work was supported by The National Key Research and Development Program of China (grant number 2018YFD1000607).

Acknowledgments

The authors are grateful to Huang Dong and Gu Bao for their suggestions for the experiment.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Huang, J.; Zhang, C.M.; Zhao, X.; Fei, Z.J.; Wan, K.K.; Zhang, Z.; Pang, X.M.; Yin, X.; Bai, Y.; Sun, X.Q.; et al. The Jujube Genome Provides Insights into Genome Evolution and the Domestication of SweetnessAcidity Taste in Fruit Trees. PLoS Genet. 2016, 12, e1006433. [Google Scholar] [CrossRef]
  2. Gao, Q.H.; Wu, C.S.; Wang, M. The Jujube (Ziziphus jujuba Mill.) Fruit: A Review of Current Knowledge of Fruit Composition and Health Benefits. J. Agric. Food Chem. 2013, 61, 3351–3363. [Google Scholar] [CrossRef]
  3. Wojdylo, A.; Carbonell-Barrachina, A.A.; Legua, P.; Hernandez, F. Phenolic composition, ascorbic acid content, and antioxidant capacity of Spanish jujube (Ziziphus jujuba Mill.) fruits. Food Chem. 2016, 201, 307–314. [Google Scholar] [CrossRef]
  4. Petroni, K.; Tonelli, C. Recent advances on the regulation of anthocyanin synthesis in reproductive organs. Plant Sci. Int. J. Exp. Plant Biol. 2011, 181, 219–229. [Google Scholar] [CrossRef]
  5. Xu, W.; Dubos, C.; Lepiniec, L. Transcriptional control of flavonoid biosynthesis by MYB-bHLH-WDR complexes. Trends Plant Sci. 2015, 20, 176–185. [Google Scholar] [CrossRef] [PubMed]
  6. Ma, P.C.M.; Rould, M.A.; Weintraub, H.; Pabo, C.O. Crystal structure of MyoD bHLH domain-DNA complex Perspectives on DNA recognition and implications for transcriptional activation. Cell 1994, 77, 451–459. [Google Scholar] [CrossRef]
  7. Abe, H.; Yamaguchi-Shinozaki, K.; Urao, T.; Iwasaki, T.; Hosokawa, D.; Shinozaki, K. Role of arabidopsis MYC and MYB homologs in drought and abscisic acid-regulated gene expression. Plant Cell 1997, 9, 1859–1868. [Google Scholar] [PubMed]
  8. Chinnusamy, V.; Ohta, M.; Kanrar, S.; Lee, B.H.; Hong, X.; Agarwal, M.; Zhu, J.K. ICE1: A regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis. Genes Dev. 2003, 17, 1043–1054. [Google Scholar] [CrossRef] [PubMed]
  9. Jiang, Y.; Deyholos, M.K. Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes. BMC Plant Biol. 2006, 6, 25. [Google Scholar] [CrossRef]
  10. Chen, S.; Yang, W.; Jia, Q.; Wang, W.; Zhang, N.; Wang, X.; Wang, S. Pleurotus ostreatus bHLH transcription factors regulate plant growth and development when expressed in Arabidopsis. J. Plant Interact. 2017, 12, 542–549. [Google Scholar] [CrossRef]
  11. Makkena, S.; Lamb, R.S. The bHLH transcription factor SPATULA is a key regulator of organ size in Arabidopsis thaliana. Plant Signal Behav. 2013, 8, e24140. [Google Scholar] [CrossRef]
  12. Hyun, Y.; Lee, I. KIDARI, encoding a non-DNA Binding bHLH protein, represses light signal transduction in Arabidopsis thaliana. Plant Mol. Biol. 2006, 61, 283–296. [Google Scholar] [CrossRef] [PubMed]
  13. Elomaa, P.; Mehto, M.; Kotilainen, M.; Helariutta, Y.; Nevalainen, L.; Teeri, T.H. A bHLH transcription factor mediates organ, region and flower type specific signals on dihydroflavonol-4-reductase (dfr) gene expression in the inflorescence of Gerbera hybrida (Asteraceae). Plant J. 1998, 16, 93–99. [Google Scholar] [CrossRef] [PubMed]
  14. Escaray, F.J.; Passeri, V.; Perea-Garcia, A.; Antonelli, C.J.; Damiani, F.; Ruiz, O.A.; Paolocci, F. The R2R3-MYB TT2b and the bHLH TT8 genes are the major regulators of proanthocyanidin biosynthesis in the leaves of Lotus species. Planta 2017, 246, 243–261. [Google Scholar] [CrossRef]
  15. Patra, B.; Pattanaik, S.; Schluttenhofer, C.; Yuan, L. A network of jasmonate-responsive bHLH factors modulate monoterpenoid indole alkaloid biosynthesis in Catharanthus roseus. New Phytol. 2018, 217, 1566–1581. [Google Scholar] [CrossRef] [PubMed]
  16. Nemesio-Gorriz, M.; Blair, P.B.; Dalman, K.; Hammerbacher, A.; Arnerup, J.; Stenlid, J.; Mukhtar, S.M.; Elfstrand, M. Identification of Norway Spruce MYB-bHLH-WDR Transcription Factor Complex Members Linked to Regulation of the Flavonoid Pathway. Front. Plant Sci. 2017, 8, 305. [Google Scholar] [CrossRef] [PubMed]
  17. Ludwig, S.R.; Habera, L.F.; Dellaporta, S.L.; Wessler, S.R. Lc, a member of the maize R gene family responsible for tissue-specific anthocyanin production, encodes a protein similar to transcriptional activators and contains the myc-homology region. Proc. Natl. Acad. Sci. USA 1989, 86, 7092–7096. [Google Scholar] [CrossRef] [PubMed]
  18. Bailey, P.C.; Martin, C.; Toledo-Ortiz, G.; Quail, P.H.; Huq, E.; Heim, M.A.; Jakoby, M.; Werber, M.; Weisshaar, B. Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana. Plant Cell 2003, 15, 2497–2502. [Google Scholar] [CrossRef] [PubMed]
  19. Rushton, P.J.; Bokowiec, M.T.; Han, S.; Zhang, H.; Brannock, J.F.; Chen, X.; Laudeman, T.W.; Timko, M.P. Tobacco transcription factors: Novel insights into transcriptional regulation in the Solanaceae. Plant Physiol. 2008, 147, 280–295. [Google Scholar] [CrossRef]
  20. Li, X.; Duan, X.; Jiang, H.; Sun, Y.; Tang, Y.; Yuan, Z.; Guo, J.; Liang, W.; Chen, L.; Yin, J.; et al. Genome-wide analysis of basic/helix-loop-helix transcription factor family in rice and Arabidopsis. Plant Physiol. 2006, 141, 1167–1184. [Google Scholar] [CrossRef]
  21. Sun, H.; Fan, H.J.; Ling, H.Q. Genome-wide identification and characterization of the bHLH gene family in tomato. BMC Genom. 2015, 16, 9. [Google Scholar] [CrossRef] [PubMed]
  22. Feller, A.; Machemer, K.; Braun, E.L.; Grotewold, E. Evolutionary and comparative analysis of MYB and bHLH plant transcription factors. Plant J. 2011, 66, 94–116. [Google Scholar] [CrossRef]
  23. Li, C.; Qiu, J.; Ding, L.; Huang, M.; Huang, S.; Yang, G.; Yin, J. Anthocyanin biosynthesis regulation of DhMYB2 and DhbHLH1 in Dendrobium hybrids petals. Plant Physiol. Biochem. 2017, 112, 335–345. [Google Scholar] [CrossRef]
  24. Xu, H.; Wang, N.; Liu, J.; Qu, C.; Wang, Y.; Jiang, S.; Lu, N.; Wang, D.; Zhang, Z.; Chen, X. The molecular mechanism underlying anthocyanin metabolism in apple using the MdMYB16 and MdbHLH33 genes. Plant Mol. Biol. 2017, 94, 149–165. [Google Scholar] [CrossRef] [PubMed]
  25. Gonzalez, A.; Zhao, M.; Leavitt, J.M.; Lloyd, A.M. Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings. Plant J. 2008, 53, 814–827. [Google Scholar] [CrossRef]
  26. Xie, X.B.; Li, S.; Zhang, R.F.; Zhao, J.; Chen, Y.C.; Zhao, Q.; Yao, Y.X.; You, C.X.; Zhang, X.S.; Hao, Y.J. The bHLH transcription factor MdbHLH3 promotes anthocyanin accumulation and fruit colouration in response to low temperature in apples. Plant Cell Environ. 2012, 35, 1884–1897. [Google Scholar] [CrossRef] [PubMed]
  27. Qi, T.; Song, S.; Ren, Q.; Wu, D.; Huang, H.; Chen, Y.; Fan, M.; Peng, W.; Ren, C.; Xie, D. The Jasmonate-ZIM-domain proteins interact with the WD-Repeat/bHLH/MYB complexes to regulate Jasmonate-mediated anthocyanin accumulation and trichome initiation in Arabidopsis thaliana. Plant Cell 2011, 23, 1795–1814. [Google Scholar] [CrossRef]
  28. Nakatsuka, T.; Haruta, K.S.; Pitaksutheepong, C.; Abe, Y.; Kakizaki, Y.; Yamamoto, K.; Shimada, N.; Yamamura, S.; Nishihara, M. Identification and characterization of R2R3-MYB and bHLH transcription factors regulating anthocyanin biosynthesis in gentian flowers. Plant Cell Physiol. 2008, 49, 1818–1829. [Google Scholar] [CrossRef] [PubMed]
  29. Shi, Q.; Zhang, Z.; Su, J.; Zhou, J.; Li, X. Comparative Analysis of Pigments, Phenolics, and Antioxidant Activity of Chinese Jujube (Ziziphus jujuba Mill.) during Fruit Development. Molecules 2018, 23, 1917. [Google Scholar] [CrossRef]
  30. Zhang, C.; Huang, J.; Li, X. Identification of appropriate reference genes for RT-qPCR analysis in Ziziphus jujuba Mill. Sci. Hortic. 2015, 197, 166–169. [Google Scholar] [CrossRef]
  31. Liu, C.; Xie, T.; Chen, C.; Luan, A.; Long, J.; Li, C.; Ding, Y.; He, Y. Genome-wide organization and expression profiling of the R2R3-MYB transcription factor family in pineapple (Ananas comosus). BMC Genom. 2017, 18, 503. [Google Scholar] [CrossRef] [PubMed]
  32. Heim, M.A.; Jakoby, M.; Werber, M.; Martin, C.; Weisshaar, B.; Bailey, P.C. The basic helix-loop-helix transcription factor family in plants: A genome-wide study of protein structure and functional diversity. Mol. Biol. Evol. 2003, 20, 735–747. [Google Scholar] [CrossRef] [PubMed]
  33. Shimada, S.; Otsuki, H.; Sakuta, M. Transcriptional control of anthocyanin biosynthetic genes in the Caryophyllales. J. Exp. Bot. 2007, 58, 957–967. [Google Scholar] [CrossRef] [PubMed]
  34. Atchley, W.R.; Fitch, W.M. A natural classification of the basic helix-loop-helix class of transcription factors. Proc. Natl. Acad. Sci. USA 1997, 94, 5172–5176. [Google Scholar] [CrossRef]
  35. Starkevic, P.; Paukstyte, J.; Kazanaviciute, V.; Denkovskiene, E.; Stanys, V.; Bendokas, V.; Siksnianas, T.; Razanskiene, A.; Razanskas, R. Expression and Anthocyanin Biosynthesis-Modulating Potential of Sweet Cherry (Prunus avium L.) MYB10 and bHLH Genes. PLoS ONE 2015, 10, e0126991. [Google Scholar] [CrossRef]
  36. Zhang, C.; Feng, R.; Ma, R.; Shen, Z.; Cai, Z.; Song, Z.; Peng, B.; Yu, M. Genome-wide analysis of basic helix-loop-helix superfamily members in peach. PLoS ONE 2018, 13, e0195974. [Google Scholar] [CrossRef] [PubMed]
  37. Mao, K.; Dong, Q.; Li, C.; Liu, C.; Ma, F. Genome Wide Identification and Characterization of Apple bHLH Transcription Factors and Expression Analysis in Response to Drought and Salt Stress. Front. Plant Sci. 2017, 8, 480. [Google Scholar] [CrossRef]
  38. Wang, R.; Zhao, P.; Kong, N.; Lu, R.; Pei, Y.; Huang, C.; Ma, H.; Chen, Q. Genome-Wide Identification and Characterization of the Potato bHLH Transcription Factor Family. Genes 2018, 9, 54. [Google Scholar] [CrossRef]
  39. Laurence, G.; Agnès, L.; Sandra, M.; Damien, L.; Marion, V.; Ton, T.; Pascal, G. MtbHLH1, a bHLH transcription factor involved in Medicago truncatula nodule vascular patterning and nodule to plant metabolic exchanges. New Phytol. 2011, 191, 391–404. [Google Scholar]
  40. Hao, Y.; Oh, E.; Choi, G.; Liang, Z.; Wang, Z. Interactions between HLH and bHLH Factors Modulate Light-Regulated Plant Development. Mol. Plant 2012, 5, 688–697. [Google Scholar] [CrossRef]
  41. Koini, M.A.; Alvey, L.; Allen, T.; Tilley, C.A.; Harberd, N.P.; Whitelam, G.C.; Franklin, K.A. High Temperature-Mediated Adaptations in Plant Architecture Require the bHLH Transcription Factor PIF4. Curr. Biol. CB 2009, 19, 408–413. [Google Scholar] [CrossRef] [PubMed]
  42. Gabriela, T.O.; Enamul, H.; Quail, P.H. The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell 2003, 15, 1749–1770. [Google Scholar]
  43. Liu, X.-F.; Yin, X.-R.; Allan, A.C.; Lin-Wang, K.; Shi, Y.-N.; Huang, Y.-J.; Ferguson, I.B.; Xu, C.-J.; Chen, K.-S. The role of MrbHLH1 and MrMYB1 in regulating anthocyanin biosynthetic genes in tobacco and Chinese bayberry (Myrica rubra) during anthocyanin biosynthesis. Plant Cell Tissue Organ Cult. PCTOC 2013, 115, 285–298. [Google Scholar] [CrossRef]
  44. Takos, A.M.; Jaffe, F.W.; Jacob, S.R.; Bogs, J.; Robinson, S.P.; Walker, A.R. Light-induced expression of a MYB gene regulates anthocyanin biosynthesis in red apples. Plant Physiol. 2006, 142, 1216–1232. [Google Scholar] [CrossRef]
  45. Wang, X.; Xu, Y.; Zhang, S.; Cao, L.; Huang, Y.; Cheng, J.; Wu, G.; Tian, S.; Chen, C.; Liu, Y.; et al. Genomic analyses of primitive, wild and cultivated citrus provide insights into asexual reproduction. Nat. Genet. 2017, 49, 765–772. [Google Scholar] [CrossRef] [PubMed]
  46. Castellarin, S.D.; Pfeiffer, A.; Sivilotti, P.; Degan, M.; Peterlunger, E.; Di Gaspero, G. Transcriptional regulation of anthocyanin biosynthesis in ripening fruits of grapevine under seasonal water deficit. Plant Cell Environ. 2007, 30, 1381–1399. [Google Scholar] [CrossRef]
  47. Gagné, S.; Cluzet, S.; Mérillon, J.-M.; Gény, L. ABA Initiates Anthocyanin Production in Grape Cell Cultures. J. Plant Growth Regul. 2010, 30, 1–10. [Google Scholar] [CrossRef]
  48. Hara, M.; Oki, K.; Hoshino, K.; Kuboi, T. Enhancement of anthocyanin biosynthesis by sugar in radish (Raphanus sativus) hypocotyl. Plant Sci. 2003, 164, 259–265. [Google Scholar] [CrossRef]
  49. Do, C.B.; Cormier, F. Effects of low nitrate and high sugar concentrations on anthocyanin content and composition of grape (Vitis vinifera L.) cell suspension. Plant Cell Rep. 1991, 9, 500–504. [Google Scholar]
  50. Sperdouli, I.; Moustakas, M. Interaction of proline, sugars, and anthocyanins during photosynthetic acclimation of Arabidopsis thaliana to drought stress. J. Plant Physiol. 2012, 169, 577–585. [Google Scholar] [CrossRef]
  51. Holcroft, D.M.; Kader, A.A. Controlled atmosphere-induced changes in pH and organic acid metabolism may affect colour stored strawberry fruit. Postharvest Biol. Technol. 1999, 17, 19–32. [Google Scholar] [CrossRef]
  52. Zhao, J.; Dixon, R.A. The ‘ins’ and ‘outs’ of flavonoid transport. Trends Plant Sci. 2010, 15, 72–80. [Google Scholar] [CrossRef] [PubMed]
  53. Poustka, F.; Irani, N.G.; Feller, A.; Lu, Y.; Pourcel, L.; Frame, K.; Grotewold, E. A trafficking pathway for anthocyanins overlaps with the endoplasmic reticulum-to-vacuole protein-sorting route in Arabidopsis and contributes to the formation of vacuolar inclusions. Plant Physiol. 2007, 145, 1323–1335. [Google Scholar] [CrossRef] [PubMed]
  54. Gomez, C.; Conejero, G.; Torregrosa, L.; Cheynier, V.; Terrier, N.; Ageorges, A. In vivo grapevine anthocyanin transport involves vesicle-mediated trafficking and the contribution of anthoMATE transporters and GST. Plant J. 2011, 67, 960–970. [Google Scholar] [CrossRef] [PubMed]
Forests 10 00346 g001 550
Figure 1. (A) Phylogenetic analysis of ZjbHLH proteins from jujube and Arabidopsis. The filled circles represent the jujube ZjbHLH transcription factor and the open circles represent the Arabidopsis bHLH transcription factor. (B) Phylogenetic analysis of ZjGL3a, ZjGL3b and ZjTT8 with selected anthocyanin pathway bHLH proteins from other plant species. The GenBank accession numbers are: Fragaria x ananassa FabHLH (AFL02463.1), Malus domestica MdbHLH3 (ADL36597.1), Petunia x hybrida PhAN1 (AAG25927.1), Ipomoea nil InIVS (BAE94394.1), Arabidopsis thaliana AtTT8 (AEE82802.1), Zea mays ZmIN1 (AAB03841.1), Petunia x hybrida PhJAF13 (AAC39455.1), Citrus sinensis CsMYC2 (ABR68793.1), Vitis vinifera VvMYCA1 (NP_001267954.1), Malus domestica MdbHLH33 (ABB84474.1) (C) Length of protein sequences, molecular weight (MW), theoretical isoelectric point (pI), aliphatic index (Ai), and grand average of hydropathicity (GRAVY) of candidate bHLHs were calculated using the Protparam Expasy tool.
Figure 1. (A) Phylogenetic analysis of ZjbHLH proteins from jujube and Arabidopsis. The filled circles represent the jujube ZjbHLH transcription factor and the open circles represent the Arabidopsis bHLH transcription factor. (B) Phylogenetic analysis of ZjGL3a, ZjGL3b and ZjTT8 with selected anthocyanin pathway bHLH proteins from other plant species. The GenBank accession numbers are: Fragaria x ananassa FabHLH (AFL02463.1), Malus domestica MdbHLH3 (ADL36597.1), Petunia x hybrida PhAN1 (AAG25927.1), Ipomoea nil InIVS (BAE94394.1), Arabidopsis thaliana AtTT8 (AEE82802.1), Zea mays ZmIN1 (AAB03841.1), Petunia x hybrida PhJAF13 (AAC39455.1), Citrus sinensis CsMYC2 (ABR68793.1), Vitis vinifera VvMYCA1 (NP_001267954.1), Malus domestica MdbHLH33 (ABB84474.1) (C) Length of protein sequences, molecular weight (MW), theoretical isoelectric point (pI), aliphatic index (Ai), and grand average of hydropathicity (GRAVY) of candidate bHLHs were calculated using the Protparam Expasy tool.
Forests 10 00346 g001
Forests 10 00346 g002 550
Figure 2. Distribution of juvenile bHLH transcription factor members on chromosomes. Chorm1.MAP–Chorm12.MAP indicates 12 chromosomes.
Figure 2. Distribution of juvenile bHLH transcription factor members on chromosomes. Chorm1.MAP–Chorm12.MAP indicates 12 chromosomes.
Forests 10 00346 g002
Forests 10 00346 g003 550
Figure 3. Jujube domain of bHLH family protein. Sequence logos of the bHLH domain by the online Gene Structure Display Server; the overall height of each stack represents the degree of conservation at this position, while the height of the letters within each stack indicates the relative frequency of the corresponding amino acids.
Figure 3. Jujube domain of bHLH family protein. Sequence logos of the bHLH domain by the online Gene Structure Display Server; the overall height of each stack represents the degree of conservation at this position, while the height of the letters within each stack indicates the relative frequency of the corresponding amino acids.
Forests 10 00346 g003
Forests 10 00346 g004 550
Figure 4. Gene expression heatmap of the complete ZjbHLHs in jujube fruits at different developmental stages. Sample names JZ1-TLH3 correspond to days 50, 90 and 110 after anthesis in the jujube cultivars ‘Junzao’ and ‘Tailihong’. The color scale shown at the top represents log2-transformed FPKM (fragments per kilobase of exon per million mapped reads) counts. Original FPKM counts are displayed in the corresponding rectangles. Red indicates high expression and blue indicates low expression. The ZjbHLHs with zero expression were eliminated at all developmental stages. The red rectangles indicate three candidate genes for anthocyanin synthesis.
Figure 4. Gene expression heatmap of the complete ZjbHLHs in jujube fruits at different developmental stages. Sample names JZ1-TLH3 correspond to days 50, 90 and 110 after anthesis in the jujube cultivars ‘Junzao’ and ‘Tailihong’. The color scale shown at the top represents log2-transformed FPKM (fragments per kilobase of exon per million mapped reads) counts. Original FPKM counts are displayed in the corresponding rectangles. Red indicates high expression and blue indicates low expression. The ZjbHLHs with zero expression were eliminated at all developmental stages. The red rectangles indicate three candidate genes for anthocyanin synthesis.
Forests 10 00346 g004
Forests 10 00346 g005 550
Figure 5. Content of anthocyanin and expression patterns of ZjGL3a, ZjGL3b and ZjTT8 in the jujube fruit cultivars ‘Junzao’ and ‘Tailihong’ at different stages (A–D). Error bars indicate SD from three replicates. Developmental stages S1–S6 correspond to 30, 50, 80, 90, 100 and 110 days after anthesis.
Figure 5. Content of anthocyanin and expression patterns of ZjGL3a, ZjGL3b and ZjTT8 in the jujube fruit cultivars ‘Junzao’ and ‘Tailihong’ at different stages (A–D). Error bars indicate SD from three replicates. Developmental stages S1–S6 correspond to 30, 50, 80, 90, 100 and 110 days after anthesis.
Forests 10 00346 g005
Forests 10 00346 g006 550
Figure 6. Anthocyanin regulatory interaction model. (a) Light, hormones, sucrose and organic acids can induce the coding of genes involved in anthocyanin synthesis. (b) Transcription. (c) Anthocyanin synthesis related genes synthesize related regulatory substances on the endoplasmic reticulum. (d) Classical anthocyanin synthesis pathway. (e) MYB can bind directly to anthocyanin-related genes and promote anthocyanin synthesis. It can also form complexes with bHLH and WDR to promote the anthocyanin synthesis. bHLH binds to the bHLH- recognition elements (BRE) region of the gene and MYB binds specifically to the MYB- recognition elements (MRE) region to promote downstream responses. (f) Plants can synthesize abscisic acid (ABA) in large quantities under water deficit and ABA can then activate the anthocyanin synthesis pathway. (g) Anthocyanin oxidative degeneration. (h) Anthocyanins are transported to the vicinity of the vacuole with the help of glutathione-S-transferase (GST) and are transported to the inside of the vacuole by the multidrug resistance-associated protein (MRP) transport channel. (i) Anthocyanins are transported into the vacuole under the action of the mate-type transporters (MATE) but an H+ concentration gradient, generated by the H+-ATPase pump, is required as a power source. (j) Anthocyanins can be engulfed in vacuoles by vesicles.
Figure 6. Anthocyanin regulatory interaction model. (a) Light, hormones, sucrose and organic acids can induce the coding of genes involved in anthocyanin synthesis. (b) Transcription. (c) Anthocyanin synthesis related genes synthesize related regulatory substances on the endoplasmic reticulum. (d) Classical anthocyanin synthesis pathway. (e) MYB can bind directly to anthocyanin-related genes and promote anthocyanin synthesis. It can also form complexes with bHLH and WDR to promote the anthocyanin synthesis. bHLH binds to the bHLH- recognition elements (BRE) region of the gene and MYB binds specifically to the MYB- recognition elements (MRE) region to promote downstream responses. (f) Plants can synthesize abscisic acid (ABA) in large quantities under water deficit and ABA can then activate the anthocyanin synthesis pathway. (g) Anthocyanin oxidative degeneration. (h) Anthocyanins are transported to the vicinity of the vacuole with the help of glutathione-S-transferase (GST) and are transported to the inside of the vacuole by the multidrug resistance-associated protein (MRP) transport channel. (i) Anthocyanins are transported into the vacuole under the action of the mate-type transporters (MATE) but an H+ concentration gradient, generated by the H+-ATPase pump, is required as a power source. (j) Anthocyanins can be engulfed in vacuoles by vesicles.
Forests 10 00346 g006
Table
Table 1. RT-qPCR primers.
Table 1. RT-qPCR primers.
Gene NameF (Primer Sequence (5′-3′))R (Primer Sequence (5′-3′))Length (bp)
ZjGL3aGCATTCTGCTGCATTGTCTCCCCCTTTTTGCCTTTATTTT194
ZjGL3bCAGCCACACCCAACCACTACACCACACCTCCCAGAAAG279
ZjTT8ATCATCACACCCGCACAGAACCCAACCAAAAGAGAACCCA114
ZjUBQTGGATGATTCTGGCAAAGGTAATGGCGGTCAAAGTG98
ZjUBQ2CACCCGTTACTTGCTTTCCTCTTCCCATTGTCCTCC93

Share and Cite

MDPI and ACS Style

Shi, Q.; Li, X.; Du, J.; Li, X. Anthocyanin Synthesis and the Expression Patterns of bHLH Transcription Factor Family during Development of the Chinese Jujube Fruit (Ziziphus jujuba Mill.). Forests 2019, 10, 346. https://doi.org/10.3390/f10040346

AMA Style

Shi Q, Li X, Du J, Li X. Anthocyanin Synthesis and the Expression Patterns of bHLH Transcription Factor Family during Development of the Chinese Jujube Fruit (Ziziphus jujuba Mill.). Forests. 2019; 10(4):346. https://doi.org/10.3390/f10040346

Chicago/Turabian Style

Shi, Qianqian, Xi Li, Jiangtao Du, and Xingang Li. 2019. "Anthocyanin Synthesis and the Expression Patterns of bHLH Transcription Factor Family during Development of the Chinese Jujube Fruit (Ziziphus jujuba Mill.)" Forests 10, no. 4: 346. https://doi.org/10.3390/f10040346

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop