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Effects of Sucrose, Boric Acid, pH, and Incubation Time on in Vitro Germination of Pollen and Tube Growth of Chinese fir (Cunnighamial lanceolata L.)

1
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Faculty of Natural Resources and Environmental Studies, University of Kordofan, El-Obeid 160, Sudan
3
State Forestry Administration Engineering Research Center of Chinese Fir, Fuzhou 350002, China
4
Key Laboratory for Forest Adversity, Physiological Ecology and Molecular Biology, Fuzhou 350002, China
5
Department of Bio-sciences, Sokoine University of Agriculture, Morogoro 3038, Tanzania
*
Author to whom correspondence should be addressed.
Forests 2019, 10(2), 102; https://doi.org/10.3390/f10020102
Received: 23 December 2018 / Revised: 10 January 2019 / Accepted: 18 January 2019 / Published: 28 January 2019
(This article belongs to the Section Forest Ecophysiology and Biology)
In vitro pollen germination provides a novel approach and strategy to accelerate genetic improvement of tree breeding. Studies about pollen germination and tube growth of Chinese fir are limited. Therefore, this study aimed to investigate the effects of sucrose, boric acid, pH, and time of incubation on pollen germination and tube growth. Pollen from 9 clones were selected. In vitro germination was performed in basic media as control, and in different concentrations of sucrose (0, 10 and 15%), boric acid (0.01, 0.1 and 0.2%), and pH levels (4.5, 5 and 7). Pollen germination rates and tube growth were recorded periodically at 1, 12, 24, and 48 h. The results showed that sucrose imposes significant effects on pollen germination and tube growth. The effects are most obvious at concentration of 15%. Boric acid significantly promoted germination and tube growth. The promotion was most notable in lower concentration of 0.01%. The media adjusted to pH 7.0 boosted the germination and pollen tube growth. The optimum time of incubation was 24 and 48 h for pollen germination and tube growth, respectively. Sucrose, pH, and time of incubation were positively correlated, whereas boric acid negatively correlated with pollen germination and tube growth. This study provided experimental evidences for selecting viable pollens for Chinese fir breeding. View Full-Text
Keywords: Chinese fir; pollen grains; in vitro germination; pollen germination; pollen tube growth Chinese fir; pollen grains; in vitro germination; pollen germination; pollen tube growth
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MDPI and ACS Style

Fragallah, S.A.D.A.; Lin, S.; Li, N.; Ligate, E.J.; Chen, Y. Effects of Sucrose, Boric Acid, pH, and Incubation Time on in Vitro Germination of Pollen and Tube Growth of Chinese fir (Cunnighamial lanceolata L.). Forests 2019, 10, 102.

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