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Open AccessArticle

Detection and Microscopy of Alnus glutinosa Pollen Fluorescence Peculiarities

1
Institute of Regional Development, Šiauliai University, 76351 Šiauliai, Lithuania
2
Institute of Life Science and Technology, Daugavpils University, LV5401 Daugavpils, Latvia
*
Author to whom correspondence should be addressed.
Forests 2019, 10(11), 959; https://doi.org/10.3390/f10110959
Received: 30 September 2019 / Revised: 19 October 2019 / Accepted: 24 October 2019 / Published: 28 October 2019
(This article belongs to the Special Issue Study of Tree Pollen and Pollination)
Alnus glutinosa is an important woody plant in Lithuanian forest ecosystems. Knowledge of fluorescence properties of black alder pollen is necessary for scientific and practical purposes. By the results of the study, we aimed to evaluate possibilities of identifying Alnus glutinosa pollen fluorescence properties by modeling ozone effect and applying two different fluorescence-based devices. To implement the experiments, black alder pollen was collected in a typical habitat during the annual flowering period in 2018–2019. There were three groups of experimental variants, which differed in the duration of exposure to ozone, conditions of pollen storage before the start of the experiment, and the exposure time. Data for pollen fluorescence analysis were collected using two methods. The microscopy method was used in order to evaluate the possibility of employing image analysis systems for investigation of pollen fluorescence. The second data collection method is related to an automatic device identifying pollen in real time, which uses the fluorescence method in the pollen recognition process. Data were assessed employing image analysis and principal component analysis (PCA) methods. Digital images of ozone-exposed pollen observed under the fluorescence microscope showed the change of the dominant green colour toward the blue spectrum. Meanwhile, the automatic detector detects more pollen whose fluorescence is at the blue light spectrum. It must be noted that assessing pollen fluorescence several months after exposure to ozone, no effect of ozone on fluorescence remains. View Full-Text
Keywords: allergenic pollen; ozone; automatic real-time device; image analysis; principal component analysis allergenic pollen; ozone; automatic real-time device; image analysis; principal component analysis
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MDPI and ACS Style

Šaulienė, I.; Šukienė, L.; Daunys, G.; Valiulis, G.; Lankauskas, A.; Kokina, I.; Gerbreders, V.; Gavarāne, I. Detection and Microscopy of Alnus glutinosa Pollen Fluorescence Peculiarities. Forests 2019, 10, 959.

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