Next Article in Journal
Effects of Carbon Black and the Presence of Static Mechanical Strain on the Swelling of Elastomers in Solvent
Previous Article in Journal
Improved Resin–Zirconia Bonding by Room Temperature Hydrofluoric Acid Etching
Article Menu

Export Article

Open AccessArticle
Materials 2015, 8(3), 867-883;

Improved Osteoblast and Chondrocyte Adhesion and Viability by Surface-Modified Ti6Al4V Alloy with Anodized TiO2 Nanotubes Using a Super-Oxidative Solution

Facultad de Odontología Mexicali, Universidad Autónoma de Baja California, Av. Zotoluca y Chinampas, s/n, Mexicali C.P. 21040, Baja California, Mexico
Instituto de Ingeniería, Universidad Autónoma de Baja California, Blvd. B. Juárez y Calle de la Normal s/n, Mexicali C.P. 21280, Baja California, Mexico
School of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
Instituto Nacional de Rehabilitación, Calz. México Xochimilco, No. 289, Arenal de Guadalupe, México C.P. 14389, D.F., Mexico
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, México C.P. 11340, D.F., Mexico
Centro Medico Ixchel, Bravo y Obregón, Mexicali C.P. 21000, Baja California, Mexico
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Carla Renata Arciola
Received: 12 December 2014 / Revised: 7 January 2015 / Accepted: 17 February 2015 / Published: 2 March 2015
(This article belongs to the Section Biomaterials)
Full-Text   |   PDF [1889 KB, uploaded 2 March 2015]   |  


Titanium (Ti) and its alloys are amongst the most commonly-used biomaterials in orthopedic and dental applications. The Ti-aluminum-vanadium alloy (Ti6Al4V) is widely used as a biomaterial for these applications by virtue of its favorable properties, such as high tensile strength, good biocompatibility and excellent corrosion resistance. TiO2 nanotube (NTs) layers formed by anodization on Ti6Al4V alloy have been shown to improve osteoblast adhesion and function when compared to non-anodized material. In his study, NTs were grown on a Ti6Al4V alloy by anodic oxidation for 5 min using a super-oxidative aqueous solution, and their in vitro biocompatibility was investigated in pig periosteal osteoblasts and cartilage chondrocytes. Scanning electron microscopy (SEM), energy dispersion X-ray analysis (EDX) and atomic force microscopy (AFM) were used to characterize the materials. Cell morphology was analyzed by SEM and AFM. Cell viability was examined by fluorescence microscopy. Cell adhesion was evaluated by nuclei staining and cell number quantification by fluorescence microscopy. The average diameter of the NTs was 80 nm. The results demonstrate improved cell adhesion and viability at Day 1 and Day 3 of cell growth on the nanostructured material as compared to the non-anodized alloy. In conclusion, this study evidences the suitability of NTs grown on Ti6Al4V alloy using a super-oxidative water and a short anodization process to enhance the adhesion and viability of osteoblasts and chondrocytes. The results warrant further investigation for its use as medical implant materials. View Full-Text
Keywords: titanium; TiO2 nanotubes; chondrocyte; osteoblasts; adhesion; tissue engineering titanium; TiO2 nanotubes; chondrocyte; osteoblasts; adhesion; tissue engineering

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Beltrán-Partida, E.; Moreno-Ulloa, A.; Valdez-Salas, B.; Velasquillo, C.; Carrillo, M.; Escamilla, A.; Valdez, E.; Villarreal, F. Improved Osteoblast and Chondrocyte Adhesion and Viability by Surface-Modified Ti6Al4V Alloy with Anodized TiO2 Nanotubes Using a Super-Oxidative Solution. Materials 2015, 8, 867-883.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top