SAXS Studies of the Endoglucanase Cel12A from Gloeophyllum trabeum Show Its Monomeric Structure and Reveal the Influence of Temperature on the Structural Stability of the Enzyme
Abstract
:1. Introduction
2. Results and Discussion
2.1. GtCel12A Is One of the Most Thermal Stable among Other Characterized Cel12A
2.2. GtCel12A Forms Monomers in Solution
Parameters | Experimental | PDB ID: 3VL8 (AaXEG) | DAM |
---|---|---|---|
Rg (Å) | 19.4 ± 0.1 | 18.25 | 18.57 |
Dmax (Å) | 60.00 | 53.09 | 56.78 |
Resolution (Å) | 32.14 | – | 32.14 |
SAXS MW (kDa) (theoretical MW = 26.17) | 28.30 | – | – |
χ | – | 1.40 | 1.47 |
3. Experimental Section
3.1. Gene Cloning, Recombinant Expression, Protein Production, Purification and Identity Confirmation
3.2. CD
3.3. Native PAGE
3.4. SAXS Studies
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Miotto, L.S.; Dos Reis, C.V.; De Oliveira Neto, M.; Polikarpov, I. SAXS Studies of the Endoglucanase Cel12A from Gloeophyllum trabeum Show Its Monomeric Structure and Reveal the Influence of Temperature on the Structural Stability of the Enzyme. Materials 2014, 7, 5202-5211. https://doi.org/10.3390/ma7075202
Miotto LS, Dos Reis CV, De Oliveira Neto M, Polikarpov I. SAXS Studies of the Endoglucanase Cel12A from Gloeophyllum trabeum Show Its Monomeric Structure and Reveal the Influence of Temperature on the Structural Stability of the Enzyme. Materials. 2014; 7(7):5202-5211. https://doi.org/10.3390/ma7075202
Chicago/Turabian StyleMiotto, Lis S., Caio V. Dos Reis, Mario De Oliveira Neto, and Igor Polikarpov. 2014. "SAXS Studies of the Endoglucanase Cel12A from Gloeophyllum trabeum Show Its Monomeric Structure and Reveal the Influence of Temperature on the Structural Stability of the Enzyme" Materials 7, no. 7: 5202-5211. https://doi.org/10.3390/ma7075202