Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces
Abstract
:1. Introduction
2. Materials and Methods
2.1. CAD/CAM Fabrication of Zirconia Specimens
2.2. Surface Treatment
2.3. Surface Characterization
2.4. Cell Culture
2.5. Confocal Laser Scanning Microscopy
2.6. Scanning Electron Microscopy
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Particle Sizes of the Powder | Coating Thickness | |||
---|---|---|---|---|
Powder | d(p) (µm) | Range (µm) | Powder | Thickness (µm) |
HAp | 93.78 | 20–180 | HAp | 36.49 |
Cu | 67.79 | 20–130 | HAp + 3wt% Cu | 32.94 |
ZnO | 2.60 | 0.5–50 | HAp + 3 wt% ZnO | 19.75 |
n = | Median | 25% Percentile | 75% Percentile | Minimum | Maximum | |
---|---|---|---|---|---|---|
untreated | 12 | 0.18 | 0.14 | 0.24 | 0.12 | 0.29 |
hydroxyapatite | 12 | 10.55 | 7.41 | 15.61 | 5.99 | 37.30 |
hydroxyapatite + ZnO | 12 | 11.75 | 9.08 | 14.95 | 6.26 | 19.68 |
hydroxyapatite + Cu | 12 | 10.47 | 9.47 | 12.80 | 8.88 | 84.59 |
n = | Median | 25% Percentile | 75% Percentile | Minimum | Maximum | |
---|---|---|---|---|---|---|
untreated | 12 | 2.99 | 1.86 | 3.51 | 1.076 | 4.318 |
hydroxyapatit | 12 | 1.08 | 0.83 | 1.82 | 0.6126 | 2.277 |
hydroxyapatite + ZnO | 12 | 1.17 | 0.90 | 1.80 | 0.80 | 2.15 |
hydroxyapatite + Cu | 12 | 0.67 | 0.49 | 1.31 | 0.08 | 1.55 |
Cell Cluture | n = | Median | 25% Percentile | 75% Percentile | Minimum | Maximum | Significance p ≤ 0.05 | |
---|---|---|---|---|---|---|---|---|
untreated | 24 h | 6 | 4.06 | 3.63 | 4.76 | 2.73 | 5.07 | <0.0001 |
72 h | 6 | 5.56 | 5.15 | 6.73 | 4.99 | 6.87 | ||
hydroxyapatite | 24 h | 6 | 1.97 | 1.45 | 2.32 | 1.40 | 2.43 | 0.2841 |
72 h | 6 | 2.42 | 2.24 | 2.77 | 2.17 | 3.01 | ||
hydroxyapatite + ZnO | 24 h | 6 | 1.97 | 1.59 | 2.33 | 1.26 | 2.81 | 0.4663 |
72 h | 6 | 2.24 | 1.95 | 3.24 | 1.66 | 3.33 | ||
hydroxyapatite + Cu | 24 h | 6 | 1.33 | 0.89 | 1.60 | 0.85 | 1.83 | 0.7891 |
72 h | 6 | 0.97 | 0.77 | 1.14 | 0.71 | 1.30 |
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Bunz, O.; Steegmann, M.-C.; Benz, K.; Testrich, H.; Quade, A.; Naumova, E.A.; Arnold, W.H.; Fricke, K.; Piwowarczyk, A.; Dittmar, T. Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces. Materials 2022, 15, 3625. https://doi.org/10.3390/ma15103625
Bunz O, Steegmann M-C, Benz K, Testrich H, Quade A, Naumova EA, Arnold WH, Fricke K, Piwowarczyk A, Dittmar T. Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces. Materials. 2022; 15(10):3625. https://doi.org/10.3390/ma15103625
Chicago/Turabian StyleBunz, Oskar, Marie-Christine Steegmann, Korbinian Benz, Holger Testrich, Antje Quade, Ella A. Naumova, Wolfgang H. Arnold, Katja Fricke, Andree Piwowarczyk, and Thomas Dittmar. 2022. "Human Gingival Fibroblast Adhesion and Proliferation on Hydroxyapatite-Coated Zirconia Abutment Surfaces" Materials 15, no. 10: 3625. https://doi.org/10.3390/ma15103625