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Article

In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts

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Department of General Chemistry, University “Politehnica” of Bucharest, 011061 Bucharest, Romania
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National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM, 060021 Bucharest, Romania
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Faculty of Horticulture, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 011464 Bucharest, Romania
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Department of Science and Engineering of Oxide Materials and Nanomaterials, University “Politehnica” of Bucharest, 011061 Bucharest, Romania
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Phytotherapy Department, Faculty of Pharmacy, Pharmacognosy, Phytochemistry, University of Medicine and Pharmacy “Carol Davila”, 020956 Bucharest, Romania
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Research Center for Environmental Protection and Waste Management, University of Bucharest, 050107 Bucharest, Romania
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Research Institute for Fruit Growing Pitesti—Mărăcineni, 110006 Pitesti-Mărăcineni, Romania
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Authors to whom correspondence should be addressed.
Academic Editor: Rosaria Anna Picca
Materials 2021, 14(8), 1845; https://doi.org/10.3390/ma14081845
Received: 26 March 2021 / Revised: 2 April 2021 / Accepted: 6 April 2021 / Published: 8 April 2021
(This article belongs to the Special Issue Advances in Biological Nanomaterials)
The aim of the current paper is the development of phytosynthesized silver nanoparticles mediated by Raphanus sativus L. extracts obtained through two extraction methods (temperature and microwave) and to test their potential application for controlling apple crops pathogens. The phytosynthesized materials were characterized by X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. All the materials were evaluated in terms of antioxidant and in vitro antimicrobial activity (against bacteria, molds, and yeast: Escherichia coli ATCC 8738, Staphylococcus aureus ATTC 25923, Pseudomonas aeruginosa ATCC 9027, Salmonella typhimurium ATCC 14028, Candida albicans ATCC 10231, Venturia inaequalis, Podosphaera leucotricha, Fusarium oxysporum ATCC 48112, Penicillium hirsutum ATCC 52323, and Aspergillus niger ATCC 15475). Considering the results obtained in the in vitro assays, formulations based on nanoparticles phytosynthesized using Raphanus sativus L. waste extracts (RS1N) were evaluated as potential antifungal agents for horticultural crops protection, against Venturia inaequalis and Podosphaera leucotricha through in vivo assays. For the DPPH assay, the inhibition (%) varied between 37.06% (for RS1N at 0.8 mg/mL concentration) and 83.72% (for RS1N at 7.2 mg/mL concentration) compared to 19.97% (for RS2N at 0.8 mg/mL) and only 28.91% (for RS2N at 7.2 mg/mL). Similar results were obtained for RS3N (85.42% inhibition at 7.2 mg/mL) compared with RS4N (21.76% inhibition at 7.2 mg/mL). Regarding the ABTS assay, the highest scavenger activity values were recorded for samples RS1N (91.43% at 1.6 mg/mL) and RS3N (96.62% at 1.6 mg/mL). View Full-Text
Keywords: phytosynthesized AgNPs; radish extracts; antifungal activity; antimicrobial activity phytosynthesized AgNPs; radish extracts; antifungal activity; antimicrobial activity
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MDPI and ACS Style

Ungureanu, C.; Fierascu, I.; Fierascu, R.C.; Costea, T.; Avramescu, S.M.; Călinescu, M.F.; Somoghi, R.; Pirvu, C. In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts. Materials 2021, 14, 1845. https://doi.org/10.3390/ma14081845

AMA Style

Ungureanu C, Fierascu I, Fierascu RC, Costea T, Avramescu SM, Călinescu MF, Somoghi R, Pirvu C. In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts. Materials. 2021; 14(8):1845. https://doi.org/10.3390/ma14081845

Chicago/Turabian Style

Ungureanu, Camelia, Irina Fierascu, Radu C. Fierascu, Teodora Costea, Sorin M. Avramescu, Mirela F. Călinescu, Raluca Somoghi, and Cristian Pirvu. 2021. "In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts" Materials 14, no. 8: 1845. https://doi.org/10.3390/ma14081845

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