Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Ethics
2.3. Experimental Studies
2.3.1. Isolation and Characterization of Cells
2.3.2. Collagen Extraction, Hydrogel Preparation, and Characterization
Rat Tail Collagen Type I Extraction
Fourier Transform Infrared Spectroscopy (FTIR)
Gel Electrophoresis
Hydrogel Preparation and OE-MSCs Encapsulation
Field Emission Scanning Electron Microscopy
Porosity Assessment
Rheological Analysis
OE-MSCs Viability and Proliferation Assessment
Osteogenic Differentiation
ECM Mineralization
Gene Expression of Bone Markers
2.4. In Vivo Study
2.4.1. Calvarial Defect Model
2.4.2. OE-MSCs Labelling and Implantation
2.4.3. Optical Imaging
2.4.4. Micro-Computed Tomography
2.4.5. Hematoxylin and Eosin (H&E) Staining
2.5. Statistical Evaluation
3. Results
3.1. Collagen Type I Extracted from Rat Tail and Characterized Next Prepared Collagen Hydrogel
3.2. OE-MSCs Isolated from Human Olfactory Mucosa and Characterized
3.3. The Increase in the Concentration of Collagen Leads to Enhance Stem Cell Viability and Proliferation of OE-MSCs
3.4. Hydrogel Properties and Biocompatibility
3.5. Collagen Concentration of 7 mg/mL Has a Storage Modulus Comparable to the Other Concentrations
3.6. The Increase in the Concentration of Collagen Hydrogel Improved Osteogenic Differentiation of OE-MSCs
3.7. Encapsulated DiI-Labeled Human OE-MSCs in Collagen Hydrogel Were Traced in Rat Defection
3.8. The Highest Bone Healing Rate of Rat Calvarial Defection Observed in the OE-Mscs Group
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Primer |
---|---|
RUNX2 | F GCCTCCAAGGTGGTAGCCC R CGTTACCCGCCATGAGAGTA |
Collagen I (Col1) | F TCCGACCTCTCTCCTCTGAA R GAGTGGGGTTATGGAGGGAT |
Osteopontin (OPN) | F GACCTGACATCCAGTACCC R GTTTCAGCACTCTGGTCATC |
Osteocalcin (OC) | F GCAAAGGTGCAGCCTTTGTG RGGCTCCCAGCCATTGATACAG |
Alkaline Phosphatase (ALP) | F GCACCTGCCTTACTAACTC RAGACACCCATCCCATCTC |
Β-actin | F CTTCCTTCCTGGGCATG R GTC TTTGCGGATGTCCAC |
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Simorgh, S.; Milan, P.B.; Saadatmand, M.; Bagher, Z.; Gholipourmalekabadi, M.; Alizadeh, R.; Hivechi, A.; Arabpour, Z.; Hamidi, M.; Delattre, C. Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering. Materials 2021, 14, 3909. https://doi.org/10.3390/ma14143909
Simorgh S, Milan PB, Saadatmand M, Bagher Z, Gholipourmalekabadi M, Alizadeh R, Hivechi A, Arabpour Z, Hamidi M, Delattre C. Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering. Materials. 2021; 14(14):3909. https://doi.org/10.3390/ma14143909
Chicago/Turabian StyleSimorgh, Sara, Peiman Brouki Milan, Maryam Saadatmand, Zohreh Bagher, Mazaher Gholipourmalekabadi, Rafieh Alizadeh, Ahmad Hivechi, Zohreh Arabpour, Masoud Hamidi, and Cédric Delattre. 2021. "Human Olfactory Mucosa Stem Cells Delivery Using a Collagen Hydrogel: As a Potential Candidate for Bone Tissue Engineering" Materials 14, no. 14: 3909. https://doi.org/10.3390/ma14143909