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In Situ Surface-Enhanced Raman Spectroscopy of Cellular Components: Theory and Experimental Results

IBF-CNR, Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Area della Ricerca CNR di Pisa, via Moruzzi 1, I-56124 Pisa, Italy
Materials 2019, 12(9), 1564; https://doi.org/10.3390/ma12091564
Received: 4 March 2019 / Revised: 17 April 2019 / Accepted: 9 May 2019 / Published: 13 May 2019
(This article belongs to the Special Issue Vibrational Spectroscopy for Biomedical Materials Analysis)
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Abstract

In the last decade, surface-enhanced Raman spectroscopy (SERS) met increasing interest in the detection of chemical and biological agents due to its rapid performance and ultra-sensitive features. Being SERS a combination of Raman spectroscopy and nanotechnology, it includes the advantages of Raman spectroscopy, providing rapid spectra collection, small sample sizes, characteristic spectral fingerprints for specific analytes. In addition, SERS overcomes low sensitivity or fluorescence interference that represents two major drawbacks of traditional Raman spectroscopy. Nanoscale roughened metal surfaces tremendously enhance the weak Raman signal due to electromagnetic field enhancement generated by localized surface plasmon resonances. In this paper, we detected label-free SERS signals for arbitrarily configurations of dimers, trimers, etc., composed of gold nanoshells (AuNSs) and applied to the mapping of osteosarcoma intracellular components. The experimental results combined to a theoretical model computation of SERS signal of specific AuNSs configurations, based on open cavity plasmonics, give the possibility to quantify SERS enhancement for overcoming spectral fluctuations. The results show that the Raman signal is locally enhanced inside the cell by AuNSs uptake and correspondent geometrical configuration generating dimers are able to enhance locally electromagnetic fields. The SERS signals inside such regions permit the unequivocal identification of cancer-specific biochemical components such as hydroxyapatite, phenylalanine, and protein denaturation due to disulfide bonds breaking between cysteine links or proline. View Full-Text
Keywords: Surface-enhanced Raman spectroscopy; plasmonic Green function; gold nanoshells AuNSs; quasi normal modes Surface-enhanced Raman spectroscopy; plasmonic Green function; gold nanoshells AuNSs; quasi normal modes
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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D’Acunto, M. In Situ Surface-Enhanced Raman Spectroscopy of Cellular Components: Theory and Experimental Results. Materials 2019, 12, 1564.

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