Next Article in Journal
Corrosion Evaluation of 316L Stainless Steel in CNT-Water Nanofluid: Effect of CNTs Loading
Previous Article in Journal
Exploiting Plasma Exposed, Natural Surface Nanostructures in Ramie Fibers for Polymer Composite Applications
Previous Article in Special Issue
Potential of Chemical and Physical Enhancers for Transungual Delivery of Amorolfine Hydrochloride
Article Menu

Export Article

Open AccessArticle

pH-Sensitive Ratiometric Fluorescent Probe for Evaluation of Tumor Treatments

1,2, 1,2, 1,2, 1,* and 1,3,*
1
Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
2
School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China
3
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
*
Authors to whom correspondence should be addressed.
Materials 2019, 12(10), 1632; https://doi.org/10.3390/ma12101632 (registering DOI)
Received: 30 April 2019 / Revised: 15 May 2019 / Accepted: 16 May 2019 / Published: 18 May 2019
(This article belongs to the Special Issue Biomaterial Design for Disease Applications)
  |  
PDF [2126 KB, uploaded 18 May 2019]
  |     |  

Abstract

Determining therapeutic efficacy is critical for tumor precision theranostics. In order to monitor the efficacy of anti-cancer drugs (e.g., Paclitaxel), a pH-sensitive ratiometric fluorescent imaging probe was constructed. The pH-sensitive ratiometric fluorescent dye ANNA was covalently coupled to the N-terminal of the cell-penetrating TAT peptide through an amidation reaction (TAT-ANNA). The in vitro cellular experiments determined that the TAT-ANNA probe could penetrate the cell membrane and image the intracellular pH in real time. The in vivo experiments were then carried out, and the ratiometric pH response to the state of the tumor was recorded immediately after medication. The TAT-ANNA probe was successfully used to monitor the pharmacodynamics of anti-cancer drugs in vivo. View Full-Text
Keywords: ratiometric fluorescence; intracellular pH; pharmacodynamic evaluation ratiometric fluorescence; intracellular pH; pharmacodynamic evaluation
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

Share & Cite This Article

MDPI and ACS Style

Zhang, P.; Meng, J.; Li, Y.; Wang, Z.; Hou, Y. pH-Sensitive Ratiometric Fluorescent Probe for Evaluation of Tumor Treatments. Materials 2019, 12, 1632.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top