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pH-Sensitive Ratiometric Fluorescent Probe for Evaluation of Tumor Treatments

1,2, 1,2, 1,2, 1,* and 1,3,*
Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
Authors to whom correspondence should be addressed.
Materials 2019, 12(10), 1632; (registering DOI)
Received: 30 April 2019 / Revised: 15 May 2019 / Accepted: 16 May 2019 / Published: 18 May 2019
(This article belongs to the Special Issue Biomaterial Design for Disease Applications)
PDF [2126 KB, uploaded 18 May 2019]
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Determining therapeutic efficacy is critical for tumor precision theranostics. In order to monitor the efficacy of anti-cancer drugs (e.g., Paclitaxel), a pH-sensitive ratiometric fluorescent imaging probe was constructed. The pH-sensitive ratiometric fluorescent dye ANNA was covalently coupled to the N-terminal of the cell-penetrating TAT peptide through an amidation reaction (TAT-ANNA). The in vitro cellular experiments determined that the TAT-ANNA probe could penetrate the cell membrane and image the intracellular pH in real time. The in vivo experiments were then carried out, and the ratiometric pH response to the state of the tumor was recorded immediately after medication. The TAT-ANNA probe was successfully used to monitor the pharmacodynamics of anti-cancer drugs in vivo. View Full-Text
Keywords: ratiometric fluorescence; intracellular pH; pharmacodynamic evaluation ratiometric fluorescence; intracellular pH; pharmacodynamic evaluation

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Zhang, P.; Meng, J.; Li, Y.; Wang, Z.; Hou, Y. pH-Sensitive Ratiometric Fluorescent Probe for Evaluation of Tumor Treatments. Materials 2019, 12, 1632.

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