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Materials 2018, 11(5), 782; https://doi.org/10.3390/ma11050782

Ureolytic/Non-Ureolytic Bacteria Co-Cultured Self-Healing Agent for Cementitious Materials Crack Repair

Department of Civil and Environmental Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea
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Received: 18 March 2018 / Revised: 21 April 2018 / Accepted: 4 May 2018 / Published: 11 May 2018
(This article belongs to the Section Advanced Composites)
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Abstract

The present study investigated the CaCO3 precipitation performance of ureolytic and non-ureolytic bacteria co-cultured as a self-healing agent for cementitious materials crack repair. Three different inoculum ratios of ureolytic Sporosarcina pasteurii and non-ureolytic Bacillus thuringiensis (10:0, 8:2, or 5:5) were used. The effect of coculturing ureolytic and non-ureolytic bacteria on microbial metabolism was investigated by measuring the rate of growth in urea-containing medium and the rate of NH4+ and CaCO3 production in urea–calcium lactate medium. The self-healing efficiency of co-cultured bacteria was examined by exposing cement mortar specimens with predefined cracks to media containing single urease-producing or co-cultured bacteria. The obtained results provide new findings, where CaCO3 precipitation is improved by co-culturing ureolytic and non-ureolytic bacteria, owing to the relatively faster growth rate of non-ureolytic bacteria. The crack filling rate correlated with the width of crack, in particular, specimens with a smaller crack width showed the faster filling effect, indicating that the crack width can be a dominant factor influencing the CaCO3 precipitation capacity of co-cultured bacteria. View Full-Text
Keywords: ureolytic bacteria; non-ureolytic bacteria; co-cultured bacteria; CaCO3 precipitation; self-healing agent ureolytic bacteria; non-ureolytic bacteria; co-cultured bacteria; CaCO3 precipitation; self-healing agent
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Son, H.M.; Kim, H.Y.; Park, S.M.; Lee, H.K. Ureolytic/Non-Ureolytic Bacteria Co-Cultured Self-Healing Agent for Cementitious Materials Crack Repair. Materials 2018, 11, 782.

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